Difference between revisions of "CcpA"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' QB5407 (spc), GP302 (erm), GP300 (an in frame deletion of ''[[ccpA]]''), available in [[Stülke]] lab; WH649 (aphA3), available in [[Gerald Seidel]]'s lab
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* '''Mutant:'''  
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** QB5407 (spc), available in [[Jörg Stülke]]'s lab
 +
** GP302 (erm), available in [[Jörg Stülke]]'s lab
 +
** GP300 (an in frame deletion of ''[[ccpA]]''), available in [[Jörg Stülke]]'s lab
 +
** WH649 (aphA3), available in [[Gerald Seidel]]'s lab
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
** pGP643 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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** pGP643 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab
 
** pWH940 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Gerald Seidel]]'s lab
 
** pWH940 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Gerald Seidel]]'s lab
 
 
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''Antibody:''' available in [[Hillen]] and [[Stülke]] labs
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* '''Antibody:''' available in [[Gerald Seidel]]'s and in [[Jörg Stülke]]'s lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 16:16, 30 October 2012

  • Description: Carbon catabolite control protein A, involved in glucose regulation of many genes; represses catabolic genes and activates genes involved in excretion of excess carbon

Gene name ccpA
Synonyms graR, alsA, amyR
Essential no
Product transcriptional regulator (LacI family)
Function mediates carbon catabolite repression (CCR)
Gene expression levels in SubtiExpress: ccpA
Interactions involving this protein in SubtInteract: CcpA
Metabolic function and regulation of this protein in SubtiPathways:
Nucleoside catabolism, Nucleotides (regulation), Ile, Leu, Val,
His, Coenzyme A, Central C-metabolism
MW, pI 36,8 kDa, 5.06
Gene length, protein length 1002 bp, 334 amino acids
Immediate neighbours motP, aroA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CcpA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CcpA expression.png
















Categories containing this gene/protein

regulators of core metabolism

This gene is a member of the following regulons

The CcpA regulon

The gene

Basic information

  • Locus tag: BSU29740

Phenotypes of a mutant

Loss of carbon catabolite repression. Loss of PTS-dependent sugar transport due to excessive phosphorylation of HPr by HprK. The mutant is unable to grow on a minimal medium with glucose and ammonium as the only sources of carbon and nitrogen, respectively.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcriptional regulator of carbon catabolite repression (CCR)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • HTH LacI-type Domain (1 – 58)
    • DNA binding Domain (6 – 25)
  • Modification:
  • Cofactor(s): HPr-Ser46-P, Crh-Ser-46-P
  • Effectors of protein activity:glucose-6-phosphate, fructose-1,6-bisphosphate Pubmed

Database entries

  • Structure:
    • 2JCG (Apoprotein from Bacillus megaterium)
    • CcpA-Crh-DNA-complex NCBI
    • complex with P-Ser-HPr and sulphate ions NCBI
    • 3OQM (complex of B. subtilis CcpA with P-Ser-HPr and the ackA operator site)
    • 3OQN (complex of B. subtilis CcpA with P-Ser-HPr and the gntR operator site)
    • 3OQO (complex of B. subtilis CcpA with P-Ser-HPr and a optimal synthetic operator site)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Additional information: there are about 3.000 molecules of CcpA per cell PubMed, this corresponds to a concentration of 3 myM (according to PubMed)

Biological materials

  • Expression vector:
  • Strep-tag construct: GP1303 ccpA-Strep (spc) in native locus, based on (pGP1389), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:

Labs working on this gene/protein

Your additional remarks

References

Reviews


General and physiological studies

Additional publications: PubMed


Global analyses (proteome, transcriptome, ChIP-chip)


Repression of target genes by CcpA

Additional publications: PubMed


Positive regulation of gene expression by CcpA

Control of CcpA activity

CcpA-DNA interaction

Functional analysis of CcpA

Structural analyses