Difference between revisions of "SucC"
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* '''Mutant:''' | * '''Mutant:''' | ||
** 1A1006 ( ''sucC''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC] | ** 1A1006 ( ''sucC''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1006&Search=1A1006 BGSC] | ||
+ | ** GP1134 (cat), available in [[Stülke]] lab | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 08:03, 30 October 2012
- Description: succinyl-CoA synthetase (beta subunit)
Gene name | sucC |
Synonyms | |
Essential | no |
Product | succinyl-CoA synthetase (beta subunit) |
Function | TCA cycle |
Gene expression levels in SubtiExpress: sucC | |
Interactions involving this protein in SubtInteract: SucC | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 41 kDa, 4.846 |
Gene length, protein length | 1155 bp, 385 aa |
Immediate neighbours | ylqH, sucD |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16090
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
- Protein family: ATP-grasp domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten FEBS Letters
- Domains:
- Modification: phosphorylation on Ser-220 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
- GTP is not accept by the enzyme FEBS Letters
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
Database entries
- Structure: 1JKJ (E. coli)
- UniProt: P80886
- KEGG entry: [3]
- E.C. number: 6.2.1.5
Additional information
- extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References