PheA

• Description: prephenate dehydratase
  
  

Gene name	pheA
Synonyms
Essential	no
Product	prephenate dehydratase
Function	biosynthesis of phenylalanine
Gene expression levels in SubtiExpress: pheA
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	31 kDa, 5.886
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	nadR, pheB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU27900

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Prephenate = phenylpyruvate + H₂O + CO₂ (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: subject to feedback inhibtion by phenylalanine PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P21203

• KEGG entry: [3]

• E.C. number: 4.2.1.51

Expression and regulation

• Operon: pheB-pheA PubMed

• Expression browser: pheA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to feedback inhibtion by phenylalanine PubMed

Biological materials

• Mutant:
  • 1A617 ( pheA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

K Trach, J A Hoch
The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.
J Bacteriol: 1989, 171(3);1362-71
[PubMed:2537815] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen
Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition.
J Bacteriol: 1966, 91(4);1594-8
[PubMed:4956345] [WorldCat.org] [DOI] (P p)