Difference between revisions of "Tkt"

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* '''Description:''' transketolase <br/><br/>
 +
 
 +
{| align="right" border="1" cellpadding="2"
 +
|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''tkt''
 +
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tktA ''
 +
|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 +
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || transketolase
 +
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17890 tkt]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Tkt Tkt]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 4.803 
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2001 bp, 667 aa
 +
|-
 +
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzC]]'', ''[[sirA]]''
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13673&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 +
|-
 +
|-
 +
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:tkt_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:tkt_expression.png|500px]]
 +
|-
 +
|}
 +
 
 +
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
 
 +
 
 +
<br/><br/><br/><br/><br/><br/>
 +
 
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[carbon core metabolism]]}},
 +
{{SubtiWiki category|[[phosphoproteins]]}}
 +
 
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[Spo0A regulon]]}}
 +
 
 +
=The gene=
 +
 
 +
=== Basic information ===
 +
 
 +
* '''Locus tag:''' BSU17890
 +
 
 +
===Phenotypes of a mutant ===
 +
 
 +
=== Database entries ===
 +
 
 +
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tkt.html]
 +
 
 +
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11247]
 +
 
 +
=== Additional information===
 +
 
 +
 
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:''' Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
 +
 
 +
* '''Protein family:''' transketolase family (according to Swiss-Prot)
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed]
 +
 
 +
* '''Cofactor(s):''' thiamine pyrophosphate
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''[[SubtInteract|Interactions]]:''' [[Tkt]]-[[Tpi]] {{PubMed|19193632}}
 +
 
 +
* '''[[Localization]]:'''
 +
 
 +
=== Database entries ===
 +
 
 +
* '''Structure:''' [[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3K95 3K95]], complex with thiamine diphosphate, from ''B. anthracis''
 +
 
 +
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45694 P45694]
 +
 
 +
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17890]
 +
 
 +
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.2.1.1 2.2.1.1]
 +
 
 +
=== Additional information===
 +
 
 +
=Expression and regulation=
 +
 
 +
* '''Operon:''' ''tkt'' {{PubMed|9068642}}
 +
 
 +
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tkt_1919861_1921864_1 tkt] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:'''
 +
 
 +
* '''Regulation:'''
 +
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 +
 
 +
* '''Regulatory mechanism:'''
 +
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 +
 
 +
* '''Additional information:'''
 +
 
 +
=Biological materials =
 +
 
 +
* '''Mutant:''' BS4530 (aphA3), available in [[Stülke]] lab
 +
 
 +
* '''Expression vector:'''
 +
** pGP94 (N-terminal Strep-tag, for [[SPINE]], expression in B. subtilis, in [[pGP380]]), available in [[Stülke]] lab
 +
** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP820, available in [[Stülke]] lab 
 +
   
 +
* '''lacZ fusion:'''
 +
 
 +
* '''GFP fusion:'''
 +
 
 +
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
 +
 
 +
* '''Antibody:'''
 +
 
 +
* '''FLAG-tag construct:''' GP1406 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
 +
 
 +
=Labs working on this gene/protein=
 +
 
 +
=Your additional remarks=
 +
 
 +
=References=
 +
==Reviews==
 +
<pubmed>9924800 </pubmed>
 +
==Original publications==
 +
<pubmed>14651647,16493705, 19603213 9068642 16143417 15375635 19193632 </pubmed>
 +
 
 +
[[Category:Protein-coding genes]]

Revision as of 20:12, 8 August 2012

  • Description: transketolase

Gene name tkt
Synonyms tktA
Essential no
Product transketolase
Function pentose phosphate pathway
Gene expression levels in SubtiExpress: tkt
Interactions involving this protein in SubtInteract: Tkt
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 72 kDa, 4.803
Gene length, protein length 2001 bp, 667 aa
Immediate neighbours ynzC, sirA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Tkt context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tkt expression.png




























Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU17890

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)
  • Protein family: transketolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s): thiamine pyrophosphate
  • Effectors of protein activity:

Database entries

  • Structure: [3K95], complex with thiamine diphosphate, from B. anthracis
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: BS4530 (aphA3), available in Stülke lab
  • Expression vector:
    • pGP94 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP820, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1406 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

G Schenk, R G Duggleby, P F Nixon
Properties and functions of the thiamin diphosphate dependent enzyme transketolase.
Int J Biochem Cell Biol: 1998, 30(12);1297-318
[PubMed:9924800] [WorldCat.org] [DOI] (P p)

Original publications

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Tkt&oldid=174529"