Difference between revisions of "SigL"
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation | ||
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+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34200 sigL] | ||
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigL SigL] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigL SigL] |
Revision as of 16:07, 7 August 2012
- Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
Gene name | sigL |
Synonyms | |
Essential | no |
Product | RNA polymerase sigma-54 factor (sigma-L) |
Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
Gene expression levels in SubtiExpress: sigL | |
Interactions involving this protein in SubtInteract: SigL | |
MW, pI | 49,5 kDa, 7.79 |
Gene length, protein length | 1308 bp, 436 amino acids |
Immediate neighbours | yvfH, yvfG |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control
This gene is a member of the following regulons
The SigL regulon
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Transcription factors activating transcription at SigL-dependent promoters
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P24219
- KEGG entry: [3]
Additional information
Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.
Transcription factors activating transcription at SigL-dependent promoters
Expression and regulation
- Operon: sigL (according to DBTBS)
- Sigma factor:
- Additional information:
Biological materials
- Mutant: GP146 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Reviews
Additional reviews: PubMed
Original publications