DnaI

• Description: primosome component (helicase loader), part of the replisome
  
  

Gene name	dnaI
Synonyms	ytxA, dnaY
Essential	yes PubMed
Product	primosome component (helicase loader)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaI
Interactions involving this protein in SubtInteract: DnaI
MW, pI	35 kDa, 5.743
Gene length, protein length	933 bp, 311 aa
Immediate neighbours	ytxB, dnaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU28980

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-DnaI PubMed, DnaC-DnaI PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization: cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed forms foci, close to oriC PubMed

Database entries

• Structure: 2K7R (N-terminal domain)

• UniProt: P06567

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dnaI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)