CwlH

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlH
Synonyms	yqeE
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	cell wall metabolism
Gene expression levels in SubtiExpress: cwlH
MW, pI	27 kDa, 9.998
Gene length, protein length	750 bp, 250 aa
Immediate neighbours	yqeF, yqeD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU25710

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_2 domain (like BlyA, CwlA, XlyA, XlyB)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54450

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon: cwlH PubMed

• Expression browser: cwlH PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909] [WorldCat.org] [DOI] (P p)