Difference between revisions of "YwlE"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' [[protein kinases and phosphatases|protein arginine phosphatase]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein arginine phosphatase]] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || dephosphorylation of [[McsB]] | |style="background:#ABCDEF;" align="center"|'''Function''' || dephosphorylation of [[McsB]] | ||
| Line 60: | Line 60: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Protein | + | * '''Catalyzed reaction/ biological activity:''' Protein arginine phosphate + H<sub>2</sub>O = protein arginine + phosphate {{PubMed|22517742}} |
| − | * '''Protein family:''' | + | * '''Protein family:''' |
* '''Paralogous protein(s):''' [[YfkJ]] | * '''Paralogous protein(s):''' [[YfkJ]] | ||
| Line 111: | Line 111: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' ''ywlE::aphA3'' | + | * '''Mutant:''' ''ywlE::aphA3'' available from [[Ulf Gerth]]'s lab |
| − | ''ywlE::tet'' | + | ''ywlE::tet'' available from [[Ulf Gerth]]'s lab |
| − | ''ywlE::spec'' | + | ''ywlE::spec'' available from [[Ulf Gerth]]'s lab |
| − | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab | + | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| Line 123: | Line 123: | ||
* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
| − | * '''Antibody:''' available in Gerth lab | + | * '''Antibody:''' available in [[Ulf Gerth]]'s lab |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| − | + | * [[Ulf Gerth]], Greifswald, Germany | |
=Your additional remarks= | =Your additional remarks= | ||
=References= | =References= | ||
| − | <pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 </pubmed> | + | <pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 18:46, 21 April 2012
- Description: protein arginine phosphatase
| Gene name | ywlE |
| Synonyms | ipc-31d |
| Essential | no |
| Product | protein arginine phosphatase |
| Function | dephosphorylation of McsB |
| MW, pI | 16 kDa, 6.681 |
| Gene length, protein length | 450 bp, 150 aa |
| Immediate neighbours | ywlF, ywlD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36930
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein arginine phosphate + H2O = protein arginine + phosphate PubMed
- Protein family:
- Paralogous protein(s): YfkJ
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: weak oligomerization, results in inactivation of YwlE PubMed
Database entries
- Structure: 1ZGG
- UniProt: P39155
- KEGG entry: [2]
- E.C. number: 3.1.3.48
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: ywlE::aphA3 available from Ulf Gerth's lab
ywlE::tet available from Ulf Gerth's lab ywlE::spec available from Ulf Gerth's lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Ulf Gerth's lab
Labs working on this gene/protein
- Ulf Gerth, Greifswald, Germany
Your additional remarks
References
