SigF

• Description: RNA polymerase forespore-specific (early) sigma factor SigF
  
  

Gene name	sigF
Synonyms	spoIIAC
Essential	no
Product	RNA polymerase forespore-specific (early) sigma factor SigF
Function	transcription of sporulation genes (late mother cell)
Interactions involving this protein in SubtInteract: SigF
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.078
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	spoVAA, spoIIAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s): SigG

Genes controlled by SigF

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigF-Fin (probably) PubMed
  • SigF-SpoIIAB
  • SigF-(RpoB-RpoC)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)

• UniProt: P07860

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation:
  • repressed by casamino acids PubMed
  • dacF: expressed during sporulation
  • spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigF regulon

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

Oleg A Igoshin, Chester W Price, Michael A Savageau
Signalling network with a bistable hysteretic switch controls developmental activation of the sigma transcription factor in Bacillus subtilis.
Mol Microbiol: 2006, 61(1);165-84
[PubMed:16824103] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Joanna Clarkson, Iain D Campbell, Michael D Yudkin
Efficient regulation of sigmaF, the first sporulation-specific sigma factor in B.subtilis.
J Mol Biol: 2004, 342(4);1187-95
[PubMed:15351644] [WorldCat.org] [DOI] (P p)

Karen Carniol, Patrick Eichenberger, Richard Losick
A threshold mechanism governing activation of the developmental regulatory protein sigma F in Bacillus subtilis.
J Biol Chem: 2004, 279(15);14860-70
[PubMed:14744853] [WorldCat.org] [DOI] (P p)

J Dworkin, R Losick
Differential gene expression governed by chromosomal spatial asymmetry.
Cell: 2001, 107(3);339-46
[PubMed:11701124] [WorldCat.org] [DOI] (P p)

P Fawcett, P Eichenberger, R Losick, P Youngman
The transcriptional profile of early to middle sporulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2000, 97(14);8063-8
[PubMed:10869437] [WorldCat.org] [DOI] (P p)

E A Campbell, S A Darst
The anti-sigma factor SpoIIAB forms a 2:1 complex with sigma(F), contacting multiple conserved regions of the sigma factor.
J Mol Biol: 2000, 300(1);17-28
[PubMed:10864495] [WorldCat.org] [DOI] (P p)

A Petersohn, S Engelmann, P Setlow, M Hecker
The katX gene of Bacillus subtilis is under dual control of sigmaB and sigmaF.
Mol Gen Genet: 1999, 262(1);173-9
[PubMed:10503549] [WorldCat.org] [DOI] (P p)

N King, O Dreesen, P Stragier, K Pogliano, R Losick
Septation, dephosphorylation, and the activation of sigmaF during sporulation in Bacillus subtilis.
Genes Dev: 1999, 13(9);1156-67
[PubMed:10323866] [WorldCat.org] [DOI] (P p)

D A Garsin, L Duncan, D M Paskowitz, R Losick
The kinase activity of the antisigma factor SpoIIAB is required for activation as well as inhibition of transcription factor sigmaF during sporulation in Bacillus subtilis.
J Mol Biol: 1998, 284(3);569-78
[PubMed:9826499] [WorldCat.org] [DOI] (P p)

D A Garsin, D M Paskowitz, L Duncan, R Losick
Evidence for common sites of contact between the antisigma factor SpoIIAB and its partners SpoIIAA and the developmental transcription factor sigmaF in Bacillus subtilis.
J Mol Biol: 1998, 284(3);557-68
[PubMed:9826498] [WorldCat.org] [DOI] (P p)

I Bagyan, L Casillas-Martinez, P Setlow
The katX gene, which codes for the catalase in spores of Bacillus subtilis, is a forespore-specific gene controlled by sigmaF, and KatX is essential for hydrogen peroxide resistance of the germinating spore.
J Bacteriol: 1998, 180(8);2057-62
[PubMed:9555886] [WorldCat.org] [DOI] (P p)

P J Lewis, T Magnin, J Errington
Compartmentalized distribution of the proteins controlling the prespore-specific transcription factor sigmaF of Bacillus subtilis.
Genes Cells: 1996, 1(10);881-94
[PubMed:9077448] [WorldCat.org] [DOI] (P p)

A Decatur, R Losick
Identification of additional genes under the control of the transcription factor sigma F of Bacillus subtilis.
J Bacteriol: 1996, 178(16);5039-41
[PubMed:8759874] [WorldCat.org] [DOI] (P p)

S Alper, A Dufour, D A Garsin, L Duncan, R Losick
Role of adenosine nucleotides in the regulation of a stress-response transcription factor in Bacillus subtilis.
J Mol Biol: 1996, 260(2);165-77
[PubMed:8764398] [WorldCat.org] [DOI] (P p)

L Duncan, S Alper, R Losick
SpoIIAA governs the release of the cell-type specific transcription factor sigma F from its anti-sigma factor SpoIIAB.
J Mol Biol: 1996, 260(2);147-64
[PubMed:8764397] [WorldCat.org] [DOI] (P p)

L Duncan, S Alper, F Arigoni, R Losick, P Stragier
Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division.
Science: 1995, 270(5236);641-4
[PubMed:7570023] [WorldCat.org] [DOI] (P p)

E J Harry, K Pogliano, R Losick
Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(12);3386-93
[PubMed:7768847] [WorldCat.org] [DOI] (P p)

P J Lewis, S R Partridge, J Errington
Sigma factors, asymmetry, and the determination of cell fate in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1994, 91(9);3849-53
[PubMed:8171000] [WorldCat.org] [DOI] (P p)

K T Min, C M Hilditch, B Diederich, J Errington, M D Yudkin
Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase.
Cell: 1993, 74(4);735-42
[PubMed:8358793] [WorldCat.org] [DOI] (P p)

L Duncan, R Losick
SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis.
Proc Natl Acad Sci U S A: 1993, 90(6);2325-9
[PubMed:8460142] [WorldCat.org] [DOI] (P p)

D Sun, P Fajardo-Cavazos, M D Sussman, F Tovar-Rojo, R M Cabrera-Martinez, P Setlow
Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters.
J Bacteriol: 1991, 173(24);7867-74
[PubMed:1744043] [WorldCat.org] [DOI] (P p)

N Illing, J Errington
Genetic regulation of morphogenesis in Bacillus subtilis: roles of sigma E and sigma F in prespore engulfment.
J Bacteriol: 1991, 173(10);3159-69
[PubMed:1902463] [WorldCat.org] [DOI] (P p)

S R Partridge, D Foulger, J Errington
The role of sigma F in prespore-specific transcription in Bacillus subtilis.
Mol Microbiol: 1991, 5(3);757-67
[PubMed:1904527] [WorldCat.org] [DOI] (P p)

J J Wu, M G Howard, P J Piggot
Regulation of transcription of the Bacillus subtilis spoIIA locus.
J Bacteriol: 1989, 171(2);692-8
[PubMed:2492512] [WorldCat.org] [DOI] (P p)

M D Yudkin
The sigma-like product of sporulation gene spoIIAC of Bacillus subtilis is toxic to Escherichia coli.
Mol Gen Genet: 1986, 202(1);55-7
[PubMed:3007937] [WorldCat.org] [DOI] (P p)