Difference between revisions of "GlcT"
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=== Basic information === | === Basic information === | ||
| − | * '''Coordinates:''' | + | * '''Coordinates:''' 1455400 - 1456254 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
Revision as of 12:12, 13 January 2009
- Description: Transcriptional antiterminator, controls expression of the ptsGHI operon
| Gene name | glcT |
| Synonyms | ykwA |
| Essential | no |
| Product | transcriptional antiterminator of the ptsGHI operon |
| Function | transcriptional antiterminator |
| MW, pI | 33,0 kDa, 7.01 |
| Gene length, protein length | 855 bp, 285 amino acids |
| Immediate neighbours | ykvZ, ptsG |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates: 1455400 - 1456254
Phenotypes of a mutant
Database entries
- DBTBS entry:
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcriptional antiterminator, RNA-binding protein, binds the ptsG RAT sequence
- Protein family: antiterminator of the BglG/ SacY family
Extended information on the protein
- Kinetic information:
- Domains:
- RNA-binding domain (N-terminal, constitutive antiterminator)
- 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)
- Modification: phosphorylation (His104)
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: [2]
- KEGG entry: [3]
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
- Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed
- Bachem S, Stülke J. (1998) Regulation of the Bacillus subtilis GlcT antiterminator protein by components of the phosphotransferase system. J Bacteriol. 180: 5319-26 PubMed
- Greenberg, D. B., Stülke, J. & Saier Jr., M. H. (2002) Domain analysis of transcriptional regulators bearing PTS-regulatory domains. Res. Microbiol. 153: 519-526. PubMed
- Langbein, I., Bachem, S. & Stülke, J. (1999) Specific interaction of the RNA binding domain of the Bacillus subtilis transcriptional antiterminator GlcT with its RNA target, RAT. J. Mol. Biol. 293: 795-805. PubMed
- Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. PubMed
- Schilling, O., Langbein, I., Müller, M., Schmalisch, M. & Stülke, J. (2004) A protein-dependent riboswitch controlling ptsGHI operon expression in Bacillus subtilis: RNA structure rather than sequence provides interaction specificity. Nucl. Acids Res. 32: 2853-2864. PubMed
- Schmalisch, M., Bachem, S. & Stülke, J. (2003) Control of the Bacillus subtilis antiterminator protein GlcT by phosphorylation: Elucidation of the phosphorylation chain leading to inactivation of GlcT. J. Biol. Chem. 278: 51108-51115. PubMed
- Stülke, J., Arnaud, M., Rapoport, G., & Martin-Verstraete, I. (1998) PRD - A protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria. Mol. Microbiol. 28: 865-874. PubMed
