CotJA

• Description: polypeptide composition of the spore coat
  
  

Gene name	cotJA
Synonyms
Essential	no
Product	unknown
Function	polypeptide composition of the spore coat
Interactions involving this protein in SubtInteract: CotJA
MW, pI	9 kDa, 9.196
Gene length, protein length	246 bp, 82 aa
Immediate neighbours	yesF, cotJB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU06890

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CotJA-CotJC PubMed

• Localization:

Database entries

• Structure:

• UniProt: Q45536

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotJA-cotJB-cotJC-yesJ-yesK PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation (SigE, SpoIIID) PubMed

• Regulatory mechanism: SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

R W Seyler, A O Henriques, A J Ozin, C P Moran
Assembly and interactions of cotJ-encoded proteins, constituents of the inner layers of the Bacillus subtilis spore coat.
Mol Microbiol: 1997, 25(5);955-66
[PubMed:9364920] [WorldCat.org] [DOI] (P p)

A O Henriques, B W Beall, K Roland, C P Moran
Characterization of cotJ, a sigma E-controlled operon affecting the polypeptide composition of the coat of Bacillus subtilis spores.
J Bacteriol: 1995, 177(12);3394-406
[PubMed:7768848] [WorldCat.org] [DOI] (P p)