Difference between revisions of "NdoA"
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| − | * '''Description:''' [[RNase]] EndoA, MazF family toxin, | + | * '''Description:''' [[RNase]] EndoA, MazF family toxin, UACAU-specific mRNA interferase <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || mRNA interferase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || plasmid partition | |style="background:#ABCDEF;" align="center"|'''Function''' || plasmid partition | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' specifically cleaves a five-base sequence, UACAU {{PubMed|21763692}} |
* '''Protein family:''' pemK family (according to Swiss-Prot) | * '''Protein family:''' pemK family (according to Swiss-Prot) | ||
| Line 125: | Line 125: | ||
=References= | =References= | ||
| − | <pubmed>15882409,17416361, 20525796</pubmed> | + | <pubmed>15882409,17416361, 20525796 21763692 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:58, 19 July 2011
- Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
| Gene name | ndoA |
| Synonyms | ydcE |
| Essential | no |
| Product | mRNA interferase |
| Function | plasmid partition |
| MW, pI | 12 kDa, 4.892 |
| Gene length, protein length | 348 bp, 116 aa |
| Immediate neighbours | ndoAI, rsbR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
Rnases, toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04660
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed
- Protein family: pemK family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Localization:
Database entries
- Structure: 1NE8
- UniProt: P96622
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for ndoA PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jung-Ho Park, Yoshihiro Yamaguchi, Masayori Inouye
Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.
FEBS Lett: 2011, 585(15);2526-32
[PubMed:21763692]
[WorldCat.org]
[DOI]
(I p)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361]
[WorldCat.org]
[DOI]
(P p)
Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409]
[WorldCat.org]
[DOI]
(P p)
