Difference between revisions of "PonA"
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| − | * '''Description:''' [[penicillin-binding protein | + | * '''Description:''' [[penicillin-binding protein]] 1A/1B <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein 1A/1B | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[penicillin-binding protein]] 1A/1B |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || bifunctional glucosyltransferase/ transpeptidase | |style="background:#ABCDEF;" align="center"|'''Function''' || bifunctional glucosyltransferase/ transpeptidase | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a ''[[mreB]]'' mutation [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] | |
| − | prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a ''[[mreB]]'' mutation [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] | + | * deletion of ''ponA'' restores growth and normal shape of a ''[[yvcK]]'' mutant on gluconeogenic carbon sources {{PubMed|21320184}} |
=== Database entries === | === Database entries === | ||
Revision as of 17:34, 15 July 2011
- Description: penicillin-binding protein 1A/1B
| Gene name | ponA |
| Synonyms | |
| Essential | no |
| Product | penicillin-binding protein 1A/1B |
| Function | bifunctional glucosyltransferase/ transpeptidase |
| MW, pI | 99 kDa, 4.752 |
| Gene length, protein length | 2742 bp, 914 aa |
| Immediate neighbours | recU, ypoC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
cell wall synthesis, cell envelope stress proteins (controlled by SigM, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22320
Phenotypes of a mutant
- prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
- deletion of ponA restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P39793
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: constitutive during vegetative growth PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
- Antibody:
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Your additional remarks
References
Reviews
Original publications
