AnsR

• Description: transcription repressor of the ansA-ansB operon
  
  

Gene name	ansR
Synonyms
Essential	no
Product	transcriptional regulator (Xre family)
Function	negative regulation of the ansA-ansB operon
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	13 kDa, 4.807
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ansA, yqxK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

AnsR regulon

The AnsR regulon:

• ansA-ansB, ansR

The gene

Basic information

• Locus tag: BSU23590

Phenotypes of a mutant

• the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q07683

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ansR PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: negative autoregulation PubMed

• Additional information:

Biological materials

• Mutant: GP1152 (del tet) available in the Stülke lab

• Expression vector:
  • expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666] [WorldCat.org] [DOI] (I e)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)