CypX

• Description: cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
  
  

Gene name	cypX
Synonyms
Essential	no
Product	cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
Function	biosynthesis of the extracellular iron chelate pulcherrimin
MW, pI	45 kDa, 5.306
Gene length, protein length	1215 bp, 405 aa
Immediate neighbours	yvnA, yvmC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

acquisition of iron, electron transport/ other, iron metabolism

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU35060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid PubMed

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3NC3 PubMed

• UniProt: O34926

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvmC-cypX Reference

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Max J Cryle, Stephen G Bell, Ilme Schlichting
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
Biochemistry: 2010, 49(34);7282-96
[PubMed:20690619] [WorldCat.org] [DOI] (I p)

R L Uffen, E Canale-Parola
Synthesis of pulcherriminic acid by Bacillus subtilis.
J Bacteriol: 1972, 111(1);86-93
[PubMed:4204912] [WorldCat.org] [DOI] (P p)

additional paper