Difference between revisions of "Methods"
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* introduction of markerfree deletions: {{PubMed|20057169}} | * introduction of markerfree deletions: {{PubMed|20057169}} | ||
| − | * generation of markerfree mutations: {{PubMed|20134232}} | + | * generation of markerfree mutations: {{PubMed|20134232}} and {{PubMed|21542786}} |
* Mechanical cell disruption and extraction of RNA from ''B. subtilis'': [[Media:SOP-RNA.pdf]] | * Mechanical cell disruption and extraction of RNA from ''B. subtilis'': [[Media:SOP-RNA.pdf]] | ||
Revision as of 14:11, 6 May 2011
Here you can find a list of methods for working with Bacillus subtilis:
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- introduction of markerfree deletions: PubMed
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
