Difference between revisions of "PrkC"

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|style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein kinase]]
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein kinase]]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || germination in response to muropeptides
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|style="background:#ABCDEF;" align="center"|'''Function''' || [[germination]] in response to muropeptides
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3PY9 3PY3] (entire extra-cellular region of PrkC from ''Staphylococcus aureus'')  {{PubMed|21208192}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34507 O34507]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34507 O34507]
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<pubmed>12399479, 12406230, 19246764,  12842463 , 18984160 </pubmed>
 
<pubmed>12399479, 12406230, 19246764,  12842463 , 18984160 </pubmed>
 
==Expression of PrkC: {{PubMed|16025310}}==
 
==Expression of PrkC: {{PubMed|16025310}}==
 
+
==Structure of PrkC==
 +
<pubmed> 21208192 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:48, 7 January 2011

Gene name prkC
Synonyms yloP
Essential no
Product protein kinase
Function germination in response to muropeptides
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 71 kDa, 4.833
Gene length, protein length 1944 bp, 648 aa
Immediate neighbours prpC, cpgA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PrkC context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

protein modification, germination, membrane proteins, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15770

Phenotypes of a mutant

  • unable to germinate in response to muropeptides PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • Paralogous protein(s):

Proteins phosphorylated by PrkC

CpgA, EF-Tu, YezB PubMed, EF-G PubMed, YwjH, GlnA, Icd, AlsD, HPr PubMed

Extended information on the protein

  • Kinetic information:
  • Domains: PASTA domain at the C-terminus (binds muropeptides) PubMed
  • Modification: phosphorylation on Thr-290 PubMed, autophosphorylation on multiple threonine residues PubMed
  • Cofactor(s):
  • Effectors of protein activity: activated by muropeptides PubMed
  • Interactions:

Database entries

  • Structure: 3PY3 (entire extra-cellular region of PrkC from Staphylococcus aureus) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP576 (spc), OMG302 (aphA3), available in Stülke lab
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP832, available in Stülke lab
    • for expression/ purification of the kinase domain from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP849, available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1001, available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP825, available in Stülke lab
  • lacZ fusion: pGP829 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Phosphorylation of PrkC

Edwige Madec, Allan Stensballe, Sven Kjellström, Lionel Cladière, Michal Obuchowski, Ole Nørregaard Jensen, Simone J Séror
Mass spectrometry and site-directed mutagenesis identify several autophosphorylated residues required for the activity of PrkC, a Ser/Thr kinase from Bacillus subtilis.
J Mol Biol: 2003, 330(3);459-72
[PubMed:12842463] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed

Targets of PrkC-dependent phosphorylation

Phsiological role of PrkC

Expression of PrkC: PubMed

Structure of PrkC

Alessia Ruggiero, Flavia Squeglia, Daniela Marasco, Roberta Marchetti, Antonio Molinaro, Rita Berisio
X-ray structural studies of the entire extracellular region of the serine/threonine kinase PrkC from Staphylococcus aureus.
Biochem J: 2011, 435(1);33-41
[PubMed:21208192] [WorldCat.org] [DOI] (I p)