YngI

• Description: aceto-acetyl-CoA ligase, may be involved in surfactin production
  
  

Gene name	yngI
Synonyms
Essential	no
Product	aceto-acetyl-CoA ligase
Function	mother cell metabolism, leucine utilization
MW, pI	61 kDa, 5.978
Gene length, protein length	1647 bp, 549 aa
Immediate neighbours	yngH, yngJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed

• Protein family:

• Paralogous protein(s): LcfA, LcfB, YhfT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31826

• KEGG entry: [3]

• E.C. number: 6.2.1.16

Expression and regulation

• Operon: yngJ-yngI-yngH-yngG-yngF-yngE PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: