NprE

• Description: extracellular neutral protease B
  
  

Gene name	nprE
Synonyms
Essential	no
Product	extracellular neutral protease B
Function	degradation of proteins
MW, pI	56 kDa, 7.481
Gene length, protein length	1563 bp, 521 aa
Immediate neighbours	yktD, ylaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Similar, but not identical, to that of thermolysin (according to Swiss-Prot)

• Protein family: peptidase M4 family (according to Swiss-Prot)

• Paralogous protein(s): NprB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 1NPC (of Bacillus cereus)

• UniProt: P68736

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nprE (according to DBTBS)

• Sigma factor:

• Regulation:
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: