YloH

• Description: omega subunit of RNA polymerase
  
  

Gene name	yloH
Synonyms
Essential	no
Product	omega subunit of RNA polymerase
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	7 kDa, 5.513
Gene length, protein length	201 bp, 67 aa
Immediate neighbours	gmk, yloI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15690

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)

• Protein family: RNA polymerase subunit omega family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: closely associated to RNA polymerase (RpoA(2)-RpoB-RpoC) PubMed

• Localization:closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons PubMed

Database entries

• Structure:

• UniProt: O35011

• KEGG entry: [2]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • present about at half the amount of core RNA polymerase PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)

L Minakhin, S Bhagat, A Brunning, E A Campbell, S A Darst, R H Ebright, K Severinov
Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly.
Proc Natl Acad Sci U S A: 2001, 98(3);892-7
[PubMed:11158566] [WorldCat.org] [DOI] (P p)