FruK

• Description: fructose-1-phosphate kinase
  
  

Gene name	fruK
Synonyms	fruB
Essential	no
Product	fructose-1-phosphate kinase
Function	fructose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 4.885
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	fruR, fruA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)

• Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2JG5 (from Staphylococcus aureus, 45% identity, 63% similarity)

• UniProt: O31714

• KEGG entry: [3]

• E.C. number: 2.7.1.56

Expression and regulation

• Operon: fruR-fruK-fruA (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced in the presence of fructose (FruR) (according to DBTBS)

• Regulatory mechanism:
  • FruR: transcription repression (according to DBTBS)

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)