Difference between revisions of "SerA"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | * '''lacZ fusion:''' | + | * '''lacZ fusion:''' pGP187 (in [[pAC7]]), available in [[Stülke]] lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
Revision as of 18:20, 29 October 2009
- Description: phosphoglycerate dehydrogenase
| Gene name | serA |
| Synonyms | |
| Essential | no |
| Product | phosphoglycerate dehydrogenase |
| Function | biosynthesis of serine |
| Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser | |
| MW, pI | 56 kDa, 5.617 |
| Gene length, protein length | 1575 bp, 525 aa |
| Immediate neighbours | ypzE, aroC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU23070
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)
- Protein family: D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: S-cysteinylation after diamide stress (C410)PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: membrane PubMed
Database entries
- Structure:
- UniProt: P35136
- KEGG entry: [3]
- E.C. number: 1.1.1.95
Additional information
Expression and regulation
- Operon: serA
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
