Difference between revisions of "PGP172"

Revision as of 18:37, 7 September 2009

pGP172: click to enlarge

pGP172 cloning region: click to enlarge

vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

sequencing primer:

@@ Line 19: / Line 19: @@
 *'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
+<pubmed> 14717704 </pubmed>
-Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) ''Mycoplasma pneumoniae'' HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. [http://www.ncbi.nlm.nih.gov/sites/entrez/14717704 PubMed]