TsaE

• Description: P-loop ATPase
  
  

Gene name	ydiB
Synonyms
Essential	no
Product	P-loop ATPase
Function	unknown
MW, pI	17 kDa, 4.597
Gene length, protein length	474 bp, 158 aa
Immediate neighbours	thiL, ydiC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05910

Phenotypes of a mutant

reduced growth rate PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: the corresponding E. coli protein is involved in the control of the activity of the Gcp homolog PubMed

• Protein family: UPF0079 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: dimerizes and oligomerizes PubMed, the corresponding E. coli protein interacts with the protein corresponding to YdiC PubMed

• Localization: cytoplasm with the excepton of the nucleoid region PubMed

Database entries

• Structure:

• UniProt: O05515

• KEGG entry: [2]

• E.C. number:

Additional information

ATPase activity is required for in vivo function of the protein PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP833, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pHW844: pGP841, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Johanna C Karst, Anne-Emmanuelle Foucher, Tracey L Campbell, Anne-Marie Di Guilmi, David Stroebel, Chand S Mangat, Eric D Brown, Jean-Michel Jault
The ATPase activity of an 'essential' Bacillus subtilis enzyme, YdiB, is required for its cellular function and is modulated by oligomerization.
Microbiology (Reading): 2009, 155(Pt 3);944-956
[PubMed:19246765] [WorldCat.org] [DOI] (P p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)