Difference between revisions of "YabT"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' GP577 (erm), available in [[Stülke]] lab |
| − | * '''Expression vector:''' | + | * '''Expression vector:''' |
| + | ** purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP391, available in [[Stülke]] lab | ||
| + | ** purification from ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP823, available in [[Stülke]] lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| + | * translational lacZ fusion (in [[pAC7]]): pGP831, available in [[Stülke]] lab | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
Revision as of 17:05, 1 September 2009
- Description: similar to Ser/Thr kinase
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Stülke lab
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
