FadA

• Description: acetyl-CoA C-acyltransferase
  
  

Gene name	fadA
Synonyms	yusK
Essential	no
Product	acetyl-CoA C-acyltransferase
Function	fatty acid degradation
MW, pI	40 kDa, 5.184
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	fadE, fadN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA (according to Swiss-Prot)

• Protein family: thiolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32177

• KEGG entry: [3]

• E.C. number: 2.3.1.16

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed in the absence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)