TrmFO

• Description: tRNA:m(5)U-54 methyltransferase, glucose-inhibited division protein
  
  

Gene name	trmFO
Synonyms	ylyC, gid
Essential	no
Product	tRNA:m(5)U-54 methyltransferase
Function	tRNA modification
MW, pI	47 kDa, 5.767
Gene length, protein length	1305 bp, 435 aa
Immediate neighbours	topA, codV
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH₂ = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD (according to Swiss-Prot)

• Protein family: TrmFO subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39815

• KEGG entry: [3]

• E.C. number: 2.1.1.74

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: