PolC

• Description: DNA polymerase III (alpha subunit)
  
  

Gene name	polC
Synonyms	mutI, dnaF, dnaP
Essential	yes PubMed
Product	DNA polymerase III (alpha subunit)
Function	DNA replication
MW, pI	162 kDa, 5.135
Gene length, protein length	4311 bp, 1437 aa
Immediate neighbours	proS, ylxS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16580

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: PolC subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P13267

• KEGG entry: [2]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Megan E Rokop, Jennifer M Auchtung, Alan D Grossman
Control of DNA replication initiation by recruitment of an essential initiation protein to the membrane of Bacillus subtilis.
Mol Microbiol: 2004, 52(6);1757-67
[PubMed:15186423] [WorldCat.org] [DOI] (P p)

E Dervyn, C Suski, R Daniel, C Bruand, J Chapuis, J Errington, L Jannière, S D Ehrlich
Two essential DNA polymerases at the bacterial replication fork.
Science: 2001, 294(5547);1716-9
[PubMed:11721055] [WorldCat.org] [DOI] (P p)