Difference between revisions of "SucD"
| Line 82: | Line 82: | ||
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1JKJ 1JKJ] (E. coli) | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1JKJ 1JKJ] (E. coli) | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P80865 P80865] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16100] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16100] | ||
| − | * '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.5 6.2.1.5] | + | * '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.5 6.2.1.5] |
=== Additional information=== | === Additional information=== | ||
Revision as of 13:46, 20 July 2009
- Description: succinyl-CoA synthetase (alpha subunit)
| Gene name | sucD |
| Synonyms | |
| Essential | no |
| Product | succinyl-CoA synthetase (alpha subunit) |
| Function | TCA cycle |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 900 bp, 300 aa |
| Immediate neighbours | sucC, dprA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU16100
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
- Protein family: succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten FEBS Letters
- Domains:
- Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
- GTP is not accept by the enzyme FEBS Letters
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
- Interactions:
- Localization:
Database entries
- Structure: 1JKJ (E. coli)
- UniProt: P80865
- KEGG entry: [3]
- E.C. number: 6.2.1.5
Additional information
The enzyme is a dimer FEBS Letters
Expression and regulation
- Sigma factor:
- Regulatory mechanism: CcpA: transcription repression
- Additional information:
Biological materials
- Mutant: GP720 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
