YwlE - Revision history

Raphael2215: /* Biological materials */

2015-07-21T13:12:31Z

‎Biological materials

Jstuelk at 09:06, 23 January 2015

2015-01-23T09:06:30Z

Jstuelk: /* References */

2014-05-18T15:06:43Z

‎References

134.76.70.252 at 09:43, 17 April 2014

2014-04-17T09:43:44Z

134.76.38.147 at 15:02, 2 April 2014

2014-04-02T15:02:55Z

Jstuelk at 07:28, 16 December 2013

2013-12-16T07:28:07Z

Fabian Commichau: /* References */

2013-07-12T07:25:09Z

‎References

Fabian Commichau: /* References */

2013-07-12T07:19:13Z

‎References

134.76.70.252 at 14:48, 16 May 2013

2013-05-16T14:48:48Z

134.76.70.252 at 11:30, 14 May 2013

2013-05-14T11:30:35Z

← Older revision		Revision as of 13:12, 21 July 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[protein kinases and phosphatases\|protein arginine phosphatase]] <br/><br/>~~	+	{{#bank: YwlE}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ywlE''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ipc-31d ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[protein kinases and phosphatases\|protein arginine phosphatase]]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| dephosphorylation of [[McsB]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36930 ywlE]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 16 kDa, 6.681~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 450 bp, 150 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ywlF]]'', ''[[ywlD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36930 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ywlE_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlE_3791805_3792257_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ywlE_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36930]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein modification]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU36930
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36930&redirect=T BSU36930]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10941]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Protein arginine phosphate + H<sub>2</sub>O = protein arginine + phosphate {{PubMed\|22517742}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[YfkJ]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[McsB]]-[[YwlE]] (dephosphorylation of [[McsB]]) {{PubMed\|20852588,21622759}}
−
−	* '''[[Localization]]:''' weak oligomerization, results in inactivation of YwlE {{PubMed\|19678837}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36930&redirect=T BSU36930]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZGG 1ZGG]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39155 P39155]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36930]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.48 3.1.3.48]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlE_3791805_3792257_-1 ywlE] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 91 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 272 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	**''ywlE::aphA3'' available from [[Ulf Gerth]]'s lab
−	**''ywlE::tet'' available from [[Ulf Gerth]]'s lab
−	**''ywlE::spec'' available from [[Ulf Gerth]]'s lab
−	** GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
−	** GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s and [[Fabian Commichau]]'s labs {{PubMed\|25610436}}
−
−	* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:''' available in [[Ulf Gerth]]'s lab
−
−	~~=Labs working on this gene/protein=~~
−	* [[Ulf Gerth]], Greifswald, Germany
−	* [[Fabian Commichau]] Göttingen, Germany
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 25610436</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 125: / Line 125: @@
 **''ywlE::tet'' available from [[Ulf Gerth]]'s lab
 **''ywlE::spec'' available from [[Ulf Gerth]]'s lab
-**GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
+** GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
-**GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s lab
+** GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s and [[Fabian Commichau]]'s labs {{PubMed|25610436}}
 * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
@@ Line 140: / Line 140: @@
 =Labs working on this gene/protein=
 * [[Ulf Gerth]], Greifswald, Germany
 * [[Fabian Commichau]] Göttingen, Germany
@@ Line 147: / Line 146: @@
 =References=
-<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 </pubmed>
+<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 25610436</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 147: / Line 147: @@
 =References=
-<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382</pubmed>
+<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 09:43, 17 April 2014
Line 116:		Line 116:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 91 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 272 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 52: / Line 52: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 90: / Line 91: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZGG 1ZGG]

@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 80: / Line 76: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 111: / Line 107: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlE_3791805_3792257_-1 ywlE] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 125: / Line 121: @@
 **''ywlE::tet'' available from [[Ulf Gerth]]'s lab
 **''ywlE::spec'' available from [[Ulf Gerth]]'s lab
-**GP1458 (''ywlE''::''aphA3''), available in [[Stülke]] lab
+**GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
-**GP1459 (''ywlE''::''tet''), available in [[Stülke]] lab
+**GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s lab
 * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
@@ Line 147: / Line 143: @@
 =References=
-<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 </pubmed>
+<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlE_3791805_3792257_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ywlE_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlE_3791805_3792257_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ywlE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36930]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlF]]'', ''[[ywlD]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36930 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36930 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:ywlE_context.gif]]