YqhY - Revision history

Jstuelk: /* Biological materials */

2015-04-06T13:04:27Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-04-02T11:42:15Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-04-02T11:40:08Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T12:57:37Z

‎Biological materials

Jstuelk: /* Biological materials */

2015-03-06T11:38:24Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T11:23:13Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:48:22Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:48:07Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:21:31Z

‎Biological materials

Dtoedter: /* Biological materials */

2015-03-06T10:00:10Z

‎Biological materials

← Older revision		Revision as of 13:04, 6 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' unknown <br/><br/>~~	+	{{#bank: YqhY}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''yqhY''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no {{PubMed\|24178028}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| unknown~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| unknown~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24330 yqhY]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=yqhY yqhY]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 14 kDa, 4.414~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 405 bp, 135 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[nusB]]'', ''[[accC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24330 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yqhY_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqhY_2529926_2530333_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:yqhY_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24330]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[proteins of unknown function]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU24330
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24330&redirect=T BSU24330]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11709]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' asp23 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[YloU]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24330&redirect=T BSU24330]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54519 P54519]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24330]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed\|7592499}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqhY_2529926_2530333_-1 yqhY] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 127 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 893 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4905 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3644 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4928 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−	** GP1468 (''yqhY''::''erm''), available in [[Jörg Stülke]]'s lab {{PubMed\|24178028}}
−	** GP1765 (''yqhY''::''cat''), available in [[Jörg Stülke]]'s lab {{PubMed\|24178028}}
−	** GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
−
−	* '''Expression vector:'''
−	** pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
−	** pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
−	** pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [[Jörg Stülke]]'s lab
−	** pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−	** GP1471 (spc, based on [[pBP43]]), available in [[Jörg Stülke]]'s lab
−
−	* '''YFP fusion:'''
−	** GP1492 (cat, available in [[Jörg Stülke]]'s lab
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''FLAG-tag construct:'''
−	** GP1481 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>17114254, 7592499 23420519 24178028 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 146: / Line 146: @@
 * '''FLAG-tag construct:'''
-** GP1481 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
+** GP1481 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 126: / Line 126: @@
 * '''Mutant:'''
 ** GP1468 (''yqhY''::''erm''), available in [[Jörg Stülke]]'s lab {{PubMed|24178028}}
 ** GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
@@ Line 138: / Line 139: @@
 * '''GFP fusion:'''
 ** GP1471 (spc, based on [[pBP43]]), available in [[Jörg Stülke]]'s lab
 * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab

@@ Line 132: / Line 132: @@
 ** pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 ** pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

← Older revision		Revision as of 11:38, 6 March 2015
Line 141:		Line 141:

	* '''FLAG-tag construct:'''		* '''FLAG-tag construct:'''
−	** GP1476 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
	** GP1481 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab		** GP1481 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab

@@ Line 130: / Line 130: @@
 * '''Expression vector:'''
 ** pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
-** pGP1325 (N-terminal His-tag,purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
+** pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''