WprA - Revision history

Jstuelk at 10:42, 19 September 2014

2014-09-19T10:42:34Z

134.76.70.252 at 09:55, 17 April 2014

2014-04-17T09:55:23Z

134.76.38.147 at 13:22, 2 April 2014

2014-04-02T13:22:37Z

Jstuelk at 16:58, 20 February 2014

2014-02-20T16:58:41Z

Jstuelk at 16:31, 16 January 2014

2014-01-16T16:31:46Z

134.76.70.252 at 15:58, 11 November 2013

2013-11-11T15:58:18Z

Jstuelk: /* References */

2013-07-13T16:18:55Z

‎References

134.76.70.252 at 12:56, 16 May 2013

2013-05-16T12:56:34Z

134.76.70.252 at 09:52, 14 May 2013

2013-05-14T09:52:37Z

134.76.70.252 at 12:30, 13 May 2013

2013-05-13T12:30:45Z

← Older revision		Revision as of 10:42, 19 September 2014
Line 1:		Line 1:
−	* '''Description:''' secreted quality control protease <br/><br/>	+	{{#bank: WprA}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''wprA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yisM ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| secreted quality control protease~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protein quality control~~
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http~~:~~//subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10770 wprA]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WprA ~~WprA]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 96 kDa, 9.58~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2682 bp, 894 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yisL]]'', ''[[yisN]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10770 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:wprA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:wprA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10770]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall/ other]]}},~~
−	~~{{SubtiWiki category\|[[proteolysis]]}},~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[YvrHb regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU10770
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10770&redirect=T BSU10770]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/wprA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11846]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** important for [[TatAY]]-[[TatCY]]-dependent export of [[YkuE]] and [[YwbN]] {{PubMed\|23180473}}
−	** involved in degradation of [[PrsA]], [[HtrA]], and [[HtrB]] {{PubMed\|24362423}}
−
−	* '''Protein family:''' peptidase S8 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[WprA]]-[[TatAY]] {{PubMed\|23180473}}
−	** [[WprA]]-[[TatCY]] {{PubMed\|23180473}}
−
−	* '''[[Localization]]:'''
−	** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
−	** membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
−	** cell wall {{PubMed\|23180473}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10770&redirect=T BSU10770]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54423 P54423]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10770]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[wprA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 wprA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|22900538}}
−
−	* '''Regulation:'''
−	** repressed by glucose ([[CcpA]]) {{PubMed\|22900538}}
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression {{PubMed\|22900538}}
−	** [[YvrHb]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
−
−	* '''Additional information:'''
−	** An [[ncRNA\|antisense RNA]] is predicted for''[[wprA]]'' {{PubMed\|20525796}}
−	** the amount of the mRNA is substantially decreased upon depletion of [[Rny\|RNase Y]] {{PubMed\|21815947}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 224 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 2052 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1A1021 ( ''wprA''::''kan''), {{PubMed\|20400548}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1021&Search=1A1021 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed> 22900538 22923395 23180473 21815947 10075409,11987133,9004506,20525796,18957862 18763711 16306698, 9687444 12028417 24362423 24115457 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 127: / Line 127: @@
 ** An [[ncRNA|antisense RNA]] is predicted for''[[wprA]]'' {{PubMed|20525796}}
 ** the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 =Biological materials =

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/wprA.html]
@@ Line 96: / Line 97: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 78: / Line 78: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 146: / Line 146: @@
 =References=
-<pubmed> 22900538 22923395 23180473 21815947 10075409,11987133,9004506,20525796,18957862 18763711 16306698, 9687444 12028417 24362423</pubmed>
+<pubmed> 22900538 22923395 23180473 21815947 10075409,11987133,9004506,20525796,18957862 18763711 16306698, 9687444 12028417 24362423 24115457 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 68: / Line 68: @@
 * '''Catalyzed reaction/ biological activity:'''
 ** important for [[TatAY]]-[[TatCY]]-dependent export of [[YkuE]] and [[YwbN]] {{PubMed|23180473}}
 * '''Protein family:''' peptidase S8 family (according to Swiss-Prot)
@@ Line 145: / Line 146: @@
 =References=
-<pubmed> 22900538 22923395 23180473 21815947 10075409,11987133,9004506,20525796,18957862 18763711 16306698, 9687444 12028417 </pubmed>
+<pubmed> 22900538 22923395 23180473 21815947 10075409,11987133,9004506,20525796,18957862 18763711 16306698, 9687444 12028417 24362423</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10770 wprA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/WprA WprA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WprA WprA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 96 kDa, 9.58

@@ Line 28: / Line 28: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:wprA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:wprA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10770]]
 |-
 |}

@@ Line 23: / Line 23: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yisL]]'', ''[[yisN]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10770 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10770 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:wprA_context.gif]]