WalK - Revision history

Jstuelk: /* Extended information on the protein */

2014-10-27T16:08:40Z

‎Extended information on the protein

Jstuelk at 13:51, 24 September 2014

2014-09-24T13:51:45Z

Jstuelk at 13:57, 17 August 2014

2014-08-17T13:57:32Z

Jstuelk: /* Original Publications */

2014-06-06T08:22:39Z

‎Original Publications

134.76.70.252 at 10:10, 17 April 2014

2014-04-17T10:10:40Z

134.76.38.147 at 15:16, 2 April 2014

2014-04-02T15:16:47Z

Jstuelk: /* Original Publications */

2014-02-06T15:36:37Z

‎Original Publications

Jstuelk: /* Expression and regulation */

2014-01-15T17:22:09Z

‎Expression and regulation

134.76.70.252 at 14:22, 18 November 2013

2013-11-18T14:22:58Z

134.76.70.252 at 15:01, 16 May 2013

2013-05-16T15:01:51Z

← Older revision		Revision as of 16:08, 27 October 2014
Line 1:		Line 1:
−	* '''Description:''' [[two-component systems\|two-component]] sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with [[cell division]] by controlling the transcription of genes for autolysins and their inhibitors <br/><br/>	+	{{#bank: WalK}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''walK''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yycG''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[two-component systems\|two-component]] sensor kinase (OmpR family)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of cell wall metabolism~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400 walK]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WalK WalK]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 69 kDa, 4.775~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1833 bp, 611 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yycH]]'', ''[[walR]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40400 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40400 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40400 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yycG_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:walK_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell wall/ other]]}},~~
−	~~{{SubtiWiki category\|[[protein modification]]}},~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU40400
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40400&redirect=T BSU40400]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yycFGHIJ-yyxA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11461]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of [[WalR]]
−
−	* '''Protein family:''' two-component sensor kinase of the OmpR family
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
−
−	* '''Modification:''' autophosphorylation on a His residue
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[WalK]]-[[WalR]]
−	** [[WalK]]-[[YycH]] {{PubMed\|21219466}},
−	** [[WalK]]-[[YycI]] {{PubMed\|21219466}}
−	** [[WalK]]-[[DivIB]] {{PubMed\|21219466}}
−	** [[WalK]]-[[FtsL]] {{PubMed\|21219466}}
−	** [[WalK]]-[[PbpB]] {{PubMed\|21219466}}
−	** [[WalK]]-[[FtsZ]] {{PubMed\|18573169}}
−
−	* '''[[Localization]]:'''
−	** cell membrane {{PubMed\|21219466}}
−	** part of the division septum in growing cells, interacts with the [[divisome]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18573169 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40400&redirect=T BSU40400]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore.do?structureId=3sl2 3SL2] (complex of the ATP binding histidine kinase domain with ATP) {{PubMed\|22751669}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45614 Q45614]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40400]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[walR]]-[[walK]]-[[yycH]]-[[yycI]]-[[walJ]]-[[htrC]]'' {{PubMed\|9829949}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 walK] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:''' expressed during vegetative growth, repressed during stationary phase [http://www.ncbi.nlm.nih.gov/sites/entrez/9829949 PubMed]
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 236 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** pGP2636, constitutive expression in ''B. subtilis'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>19019149,18792692,10094672, 18245295 </pubmed>~~
−
−	~~==Original Publications==~~
−	~~<pubmed>18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157, 19342776 20057515 20059685 20167622 21219466 23632918 22751669,22526318 24490131 24900578 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 13:51, 24 September 2014
Line 138:		Line 138:

	* '''Expression vector:'''		* '''Expression vector:'''
		+	** pGP2636, constitutive expression in ''B. subtilis'' (based on [[pBQ200]]), available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 92: / Line 92: @@
 * '''[[SubtInteract|Interactions]]:'''
-** [[WalK]]-[[WalR]], [[WalK]]-[[YycH]] {{PubMed|21219466}}, [[WalK]]-[[YycI]] {{PubMed|21219466}}, [[WalK]]-[[DivIB]] {{PubMed|21219466}}, [[WalK]]-[[FtsL]] {{PubMed|21219466}}, [[WalK]]-[[PbpB]] {{PubMed|21219466}}
+** [[WalK]]-[[WalR]]
 * '''[[Localization]]:'''

@@ Line 150: / Line 150: @@
 ==Original Publications==
-<pubmed>18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157, 19342776 20057515 20059685 20167622 21219466 23632918 22751669,22526318 24490131 </pubmed>
+<pubmed>18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157, 19342776 20057515 20059685 20167622 21219466 23632918 22751669,22526318 24490131 24900578 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 10:10, 17 April 2014
Line 125:		Line 125:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 236 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 61: / Line 61: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yycFGHIJ-yyxA.html]
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 112: / Line 112: @@
 =Expression and regulation=
-* '''Operon:''' ''[[walR]]-[[walK]]-[[yycH]]-[[yycI]]-[[walJ]]-[[yyxA]]'' {{PubMed|9829949}}
+* '''Operon:''' ''[[walR]]-[[walK]]-[[yycH]]-[[yycI]]-[[walJ]]-[[htrC]]'' {{PubMed|9829949}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 walK] {{PubMed|22383849}}

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400 walK]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/WalK WalK]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=WalK WalK]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 69 kDa, 4.775

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:walK_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=walK_4151853_4153688_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:walK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40400]]
 |-
 |}