UvrB - Revision history

Jstuelk at 07:19, 3 March 2015

2015-03-03T07:19:22Z

134.76.38.147 at 14:54, 2 April 2014

2014-04-02T14:54:45Z

Jstuelk: /* Original publications */

2014-03-08T16:36:00Z

‎Original publications

Jstuelk at 18:46, 17 November 2013

2013-11-17T18:46:17Z

134.76.70.252 at 09:26, 12 November 2013

2013-11-12T09:26:44Z

Jstuelk at 17:07, 13 September 2013

2013-09-13T17:07:31Z

134.76.70.252 at 14:41, 16 May 2013

2013-05-16T14:41:27Z

134.76.70.252 at 11:23, 14 May 2013

2013-05-14T11:23:29Z

134.76.70.252 at 13:58, 13 May 2013

2013-05-13T13:58:06Z

134.76.70.252 at 16:27, 7 August 2012

2012-08-07T16:27:32Z

← Older revision		Revision as of 07:19, 3 March 2015
Line 1:		Line 1:
−	* '''Description:~~''' excinuclease ABC (subunit B) <br/><br/>~~	+	{{#bank: UvrB}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''uvrB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''dinA, uvrA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| excinuclease ABC (subunit B)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| DNA repair after UV damage~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35170 uvrB]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UvrB UvrB]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 76 kDa, 5.26~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1983 bp, 661 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[uvrA]]'', ''[[csbA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35170 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:uvrB_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=uvrB_3612945_3614930_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:uvrB_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35170]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA repair/ recombination]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[LexA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU35170
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35170&redirect=T BSU35170]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uvrBA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10502]
−
−	~~=== Additional information===~~
−	* A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed\|21821766}}
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' uvrB family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[UvrA]]-[[UvrB]] {{PubMed\|16464004}}
−	** [[UvrB]]-[[UvrC]] {{PubMed\|16464004}}
−	** [[UvrB]] interacts sequentially with [[UvrA]] and [[UvrC]], a complex of the three proteins was never observed {{PubMed\|16464004}}
−	** [[UvrB]]-[[PcrA]] {{PubMed\|24147116}}
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35170&redirect=T BSU35170]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2NMV 2NMV] (bound to fluorescein-adducted DNA); [http://www.rcsb.org/pdb/explore.do?structureId=2D7D 2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP {{PubMed\|16426634}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P37954 P37954]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35170]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[uvrB]]-[[uvrA]]'' (according to [http://dbtbs.hgc.jp/COG/prom/uvrBA.html DBTBS])
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=uvrB_3612945_3614930_-1 uvrB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** induced upon DNA damage ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8226626 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8226626 PubMed]
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' GP1175 (del ''uvrAB''::''ermC'') (available in the [[Stülke]] lab)
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>16464004 15927210 7801120 22933559 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>8226626, 9555905 16426634 21821766 24147116 21145481 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 54: / Line 54: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uvrBA.html]
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2NMV 2NMV] (bound to fluorescein-adducted DNA); [http://www.rcsb.org/pdb/explore.do?structureId=2D7D 2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP {{PubMed|16426634}}

@@ Line 142: / Line 142: @@
 <pubmed>16464004 15927210 7801120  22933559 </pubmed>
 == Original publications ==
-<pubmed>8226626, 9555905 16426634 21821766 24147116</pubmed>
+<pubmed>8226626, 9555905 16426634 21821766 24147116 21145481 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 86: / Line 86: @@
 * '''[[SubtInteract|Interactions]]:'''
-**  [[UvrA]]-[[UvrB]] {{PubMed|16464004}}
+** [[UvrA]]-[[UvrB]] {{PubMed|16464004}}
 ** [[UvrB]]-[[UvrC]] {{PubMed|16464004}}
 ** [[UvrB]] interacts sequentially with [[UvrA]] and [[UvrC]], a complex of the three proteins was never observed {{PubMed|16464004}}
 * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
@@ Line 141: / Line 142: @@
 <pubmed>16464004 15927210 7801120  22933559 </pubmed>
 == Original publications ==
-<pubmed>8226626, 9555905 16426634 21821766</pubmed>
+<pubmed>8226626, 9555905 16426634 21821766 24147116</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35170 uvrB]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/UvrB UvrB]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UvrB UvrB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 5.26

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 113: / Line 109: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=uvrB_3612945_3614930_-1 uvrB] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 143: / Line 139: @@
 =References=
 ==Reviews==
-<pubmed>16464004 15927210 7801120  </pubmed>
+<pubmed>16464004 15927210 7801120  22933559 </pubmed>
-==Original Publications==
+== Original publications ==
 <pubmed>8226626, 9555905 16426634 21821766</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=uvrB_3612945_3614930_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:uvrB_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=uvrB_3612945_3614930_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:uvrB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35170]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[uvrA]]'', ''[[csbA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35170 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35170 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:uvrB_context.gif]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair after UV damage
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/UvrB UvrB]