UgtP - Revision history

Jstuelk at 14:18, 19 August 2014

2014-08-19T14:18:14Z

134.76.38.147 at 14:00, 2 April 2014

2014-04-02T14:00:48Z

Jstuelk at 13:25, 30 January 2014

2014-01-30T13:25:09Z

134.76.70.252 at 11:02, 7 January 2014

2014-01-07T11:02:45Z

Jstuelk at 18:16, 21 December 2013

2013-12-21T18:16:50Z

Jstuelk: /* Expression and regulation */

2013-12-14T17:27:54Z

‎Expression and regulation

Jstuelk at 17:24, 14 December 2013

2013-12-14T17:24:59Z

134.76.70.252 at 08:40, 12 November 2013

2013-11-12T08:40:55Z

Fmehne: /* Biological materials */

2013-08-13T16:27:52Z

‎Biological materials

Fmehne: /* Biological materials */

2013-08-13T15:43:09Z

‎Biological materials

← Older revision		Revision as of 14:18, 19 August 2014
Line 1:		Line 1:
−	* '''Description:~~''' UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of [[cell division]] <br/><br/>~~	+	{{#bank: UgtP}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''ugtP''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ypfP ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| UDP-glucose diacylglycerol glucosyltransferase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| synthesis of glycolipids and anchoring of lipoteichoic <br/>acid, inhibition of [[FtsZ]] assembly~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21920 ugtP]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UgtP UgtP]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ugtP ugtP]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 43 kDa, 8.398~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1146 bp, 382 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[metA]]'', ''[[cspD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU21920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU21920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU21920 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:ugtP_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:ugtP_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21920]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[cell division]]}},~~
−	~~{{SubtiWiki category\|[[lipid metabolism/ other]]}},~~
−	~~{{SubtiWiki category\|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigM regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU21920
−
−	~~===Phenotypes of a mutant ===~~
−	* cells are bent and distended {{PubMed\|22362028}}
−	* increased expression of the [[SigM]], [[SigV]], and [[SigX]] regulons {{PubMed\|22362028}}
−	* altered [[localization]] of [[MreB]] (irregular clusters instead of helical dots) {{PubMed\|22362028}}
−	* the inactivation of ''[[ugtP]]'' suppresses the poor and filametous growth of the ''[[yvcL]] [[zapA]]'' double mutant {{PubMed\|24097947}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU21920&redirect=T BSU21920]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11611]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)
−	** the interaction with [[FtsZ]] results in inhibition of [[cell division]] and an increase of cell size {{PubMed\|22931116}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−	** oligomerization of [[UgtP]] is inhibited by UDP-Glc and by interaction with [[FtsZ]] {{PubMed\|22931116}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[EzrA]]-[[UgtP]] {{PubMed\|17662947}}
−	** [[UgtP]]-[[UgtP]] {{PubMed\|17662947}}, forms oligomers {{PubMed\|22931116}}
−	** [[UgtP]]-[[FtsZ]] (under nutrient rich conditions) {{PubMed\|22931116}}
−
−	* '''[[Localization]]:'''
−	** membrane-bound protein, self-assembles into tightly wound spirals ''in vitro'' {{PubMed\|17662947}}
−	** under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [[FtsZ]] {{PubMed\|22931116}}
−	** under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [[FtsZ]] {{PubMed\|22931116}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU21920&redirect=T BSU21920]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P54166 P54166]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU21920]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−	** ''[[metA]]-[[ugtP]]'' {{PubMed\|22383849}}
−	** ''[[ugtP]]'' {{PubMed\|22383849}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 ugtP] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−	** ''[[ugtP]]'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP1369 (''[[ugtP]]''::''spc''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−	** pGP2571, for expression in ''B. subtilis'' (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab
−	** pGP2600, for expression/ purification from ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~==Reviews==~~
−	~~<pubmed> 19680248 17662935 22575476 </pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116 24097947</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 106: / Line 107: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 69: / Line 69: @@
 === Additional information===
 =The protein=
@@ Line 142: / Line 141: @@
 * '''Expression vector:'''
 ** pGP2571, for expression in ''B. subtilis'' (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab
-** pGP2600, for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab
+** pGP2600, for expression/ purification from ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UgtP UgtP]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ugtP ugtP]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 8.398

@@ Line 57: / Line 57: @@
 ===Phenotypes of a mutant ===
-** cells are bent and distended {{PubMed|22362028}}
+* cells are bent and distended {{PubMed|22362028}}
-** increased expression of the [[SigM]], [[SigV]], and [[SigX]] regulons {{PubMed|22362028}}
+* increased expression of the [[SigM]], [[SigV]], and [[SigX]] regulons {{PubMed|22362028}}
-** altered [[localization]] of [[MreB]] (irregular clusters instead of helical dots)  {{PubMed|22362028}}
+* altered [[localization]] of [[MreB]] (irregular clusters instead of helical dots)  {{PubMed|22362028}}
 === Database entries ===
@@ Line 160: / Line 161: @@
 <pubmed> 19680248 17662935 22575476 </pubmed>
 ==Original Publications==
-<pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116</pubmed>
+<pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116 24097947</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 120: / Line 120: @@
 * '''Operon:'''
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 ugtP] {{PubMed|22383849}}
-* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''

@@ Line 39: / Line 39: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 86: / Line 86: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 104: / Line 104: @@
 ** under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [[FtsZ]]  {{PubMed|22931116}}
 ** under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [[FtsZ]]  {{PubMed|22931116}}
 === Database entries ===
@@ Line 122: / Line 123: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 ugtP] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
+* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
 * '''Regulation:'''
@@ Line 136: / Line 137: @@
 * '''Expression vector:'''
-**pGP2571 (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab
+** pGP2571, for expression in ''B. subtilis'' (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
@@ Line 154: / Line 156: @@
 ==Reviews==
 <pubmed> 19680248 17662935 22575476 </pubmed>
 ==Original Publications==
 <pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU21920 ugtP]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/UgtP UgtP]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UgtP UgtP]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''

← Older revision		Revision as of 16:27, 13 August 2013
Line 136:		Line 136:

	* '''Expression vector:'''		* '''Expression vector:'''
		+	**pGP2571 (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:'''		* '''lacZ fusion:'''

@@ Line 132: / Line 132: @@
 =Biological materials =
 * '''Mutant:'''
 * '''Expression vector:'''