TrxA - Revision history

134.76.70.252 at 14:16, 17 April 2014

2014-04-17T14:16:10Z

134.76.70.252 at 09:34, 17 April 2014

2014-04-17T09:34:59Z

134.76.38.147 at 14:27, 2 April 2014

2014-04-02T14:27:04Z

Jstuelk at 09:28, 16 January 2014

2014-01-16T09:28:08Z

Jstuelk at 09:49, 10 January 2014

2014-01-10T09:49:09Z

134.76.70.252 at 09:04, 12 November 2013

2013-11-12T09:04:24Z

Jstuelk at 11:32, 1 August 2013

2013-08-01T11:32:59Z

Jstuelk at 11:28, 1 August 2013

2013-08-01T11:28:02Z

134.76.70.252 at 14:12, 16 May 2013

2013-05-16T14:12:42Z

134.76.70.252 at 10:56, 14 May 2013

2013-05-14T10:56:34Z

← Older revision		Revision as of 14:16, 17 April 2014
Line 1:		Line 1:
−	* '''Description:''' thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange, important for the prevention of protein aggregation during severe heat stress <br/><br/>	+	{{#bank: TrxA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''trxA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''trx ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| thioredoxin~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protection of proteins against oxidative damage~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28500 trxA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TrxA TrxA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 11 kDa, 4.308~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 312 bp, 104 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[uvrC]]'', ''[[abf2]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28500 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28500 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28500 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:trxA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trxA_2913024_2913338_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:trxA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28500]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[electron transport/ other]]}},~~
−	~~{{SubtiWiki category\|[[general stress proteins (controlled by SigB)]]}},~~
−	~~{{SubtiWiki category\|[[resistance against oxidative and electrophile stress]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CtsR regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigB regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[Spx regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU28500
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28500&redirect=T BSU28500]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trxA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10348]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' thioredoxin family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[ArsC]]-[[TrxA]] {{PubMed\|17303556}}
−
−	* '''[[Localization]]:''' cytoplasm {{PubMed\|17501922}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28500&redirect=T BSU28500]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2GZZ 2GZZ] (oxidized form), [http://www.rcsb.org/pdb/explore.do?structureId=2GZY 2GZY] (reduced form), [http://www.rcsb.org/pdb/explore.do?structureId=2IPA 2IPA] (TrxA-ArsC-complex) {{PubMed\|17303556}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P14949 P14949]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28500]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''trxA'' {{PubMed\|9537387}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trxA_2913024_2913338_-1 trxA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|9537387}}, [[SigB]] {{PubMed\|9537387,11544224}}
−
−	* '''Regulation:'''
−	** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
−	** regulation by [[Spx]] in response to diamide stress
−	** induced by heat stress ([[CtsR]]) {{PubMed\|9852015}}
−
−	* '''Regulatory mechanism:'''
−	** [[CtsR]]: transcription repression {{PubMed\|9852015}}
−
−	* '''Additional information:'''
−	** A [[ncRNA]] is predicted between ''[[trxA]]'' and ''[[abf2]]'' {{PubMed\|20525796}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 7758 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 15381 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 23899494 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 24401092 24417481 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 129: / Line 129: @@
 * '''Additional information:'''
 ** A [[ncRNA]] is predicted between ''[[trxA]]'' and ''[[abf2]]'' {{PubMed|20525796}}
 =Biological materials =

@@ Line 63: / Line 63: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trxA.html]
@@ Line 98: / Line 99: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2GZZ 2GZZ] (oxidized form),  [http://www.rcsb.org/pdb/explore.do?structureId=2GZY 2GZY] (reduced form),  [http://www.rcsb.org/pdb/explore.do?structureId=2IPA 2IPA] (TrxA-ArsC-complex) {{PubMed|17303556}}

@@ Line 1: / Line 1: @@
-* '''Description:''' thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange <br/><br/>
+* '''Description:''' thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange, important for the prevention of protein aggregation during severe heat stress <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 150: / Line 150: @@
 <pubmed> 23899494 </pubmed>
 == Original publications ==
-<pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 24401092 </pubmed>
+<pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 24401092 24417481 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 84: / Line 84: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 150: / Line 150: @@
 <pubmed> 23899494 </pubmed>
 == Original publications ==
-<pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 </pubmed>
+<pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 24401092 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28500 trxA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/TrxA TrxA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TrxA TrxA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 4.308

← Older revision		Revision as of 11:32, 1 August 2013
Line 1:		Line 1:
−	* '''Description:''' antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange <br/><br/>	+	* '''Description:''' thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange <br/><br/>

	{\| align="right" border="1" cellpadding="2"		{\| align="right" border="1" cellpadding="2"

@@ Line 39: / Line 39: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 119: / Line 115: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trxA_2913024_2913338_-1 trxA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|9537387}}, [[SigB]] {{PubMed|9537387,11544224}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9537387}}, [[SigB]] {{PubMed|9537387,11544224}}
 * '''Regulation:'''
@@ Line 151: / Line 147: @@
 =References=
 <pubmed>9537387,15937154,18601268,18456801,9852015 ,11544224,18687074 20084284 17303556 12642660 20525796 17501922 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trxA_2913024_2913338_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:trxA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trxA_2913024_2913338_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:trxA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28500]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[uvrC]]'', ''[[abf2]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28500 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28500 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28500 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28500 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28500 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28500 DNA_with_flanks]
 |-
 |-