TrpS - Revision history

134.76.70.252 at 09:46, 17 April 2014

2014-04-17T09:46:20Z

134.76.38.147 at 13:24, 2 April 2014

2014-04-02T13:24:43Z

134.76.70.252 at 10:30, 7 January 2014

2014-01-07T10:30:56Z

134.76.70.252 at 12:58, 16 May 2013

2013-05-16T12:58:35Z

134.76.70.252 at 09:54, 14 May 2013

2013-05-14T09:54:34Z

134.76.70.252 at 12:32, 13 May 2013

2013-05-13T12:32:32Z

Raphael2215 at 11:04, 9 August 2012

2012-08-09T11:04:50Z

134.76.70.252 at 16:43, 6 August 2012

2012-08-06T16:43:30Z

134.76.70.252 at 17:29, 18 April 2012

2012-04-18T17:29:33Z

134.76.70.252 at 17:45, 12 April 2012

2012-04-12T17:45:07Z

← Older revision		Revision as of 09:46, 17 April 2014
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−	* '''Description:''' tryptophanyl-tRNA synthetase <br/><br/>	+	{{#bank: TrpS}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''trpS''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| tryptophanyl-tRNA synthetase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| translation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420 trpS]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=trpS trpS]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 37 kDa, 6.542~~
−	\|-
−	~~\|style="background:~~#~~ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 990 bp, 330 aa~~
−	\|-
−	~~\|style="background~~:~~#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yjbA]]'', ''[[oppA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11420 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:trpS_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:trpS_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[translation]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[T-box]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU11420
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpS.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10799]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
−
−	* '''Protein family:''' class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
−
−	* '''Structure:'''
−	** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PRH 3PRH] (Val144Pro mutant) {{PubMed\|21224416}}
−	** [http://www.rcsb.org/pdb/explore.do?structureId=1BM2 1BM2] (complex with tryptophan, ''Geobacillus stearothermophilus'')
−	** [http://www.rcsb.org/pdb/explore.do?structureId=1MAW 1MAW] (complex with ATP, ''Geobacillus stearothermophilus'')
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P21656 P21656]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11420]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.2 6.1.1.2]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''trpS'' {{PubMed\|8915527}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 trpS] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−	** induced by tryptophan limitation ([[T-box]]) {{PubMed\|8915527}}
−
−	* '''Regulatory mechanism:'''
−	** [[T-box]]: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) {{PubMed\|8915527}}
−
−	* '''Additional information:'''
−	** A [[ncRNA]] is predicted between ''[[trpS]]'' and ''[[oppA]]'' {{PubMed\|20525796}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>19258532,8932308,8915527, 21224416 20525796</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 60: / Line 60: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpS.html]
@@ Line 94: / Line 95: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420 trpS]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=trpS trpS]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 6.542

@@ Line 28: / Line 28: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:trpS_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:trpS_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11420]]
 |-
 |}

@@ Line 23: / Line 23: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbA]]'', ''[[oppA]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11420 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11420 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:trpS_context.gif]]

@@ Line 1: / Line 1: @@
+* '''Description:''' tryptophanyl-tRNA synthetase <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-

@@ Line 103: / Line 103: @@
 * '''Operon:''' ''trpS'' {{PubMed|8915527}}
-* '''[[Sigma factor]]:'''
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 trpS] {{PubMed|22383849}}
 * '''Regulation:'''