Tgl - Revision history

134.76.38.147 at 14:37, 2 April 2014

2014-04-02T14:37:05Z

Jstuelk at 16:12, 13 August 2013

2013-08-13T16:12:57Z

Jstuelk at 15:44, 13 August 2013

2013-08-13T15:44:02Z

Jstuelk at 15:36, 13 August 2013

2013-08-13T15:36:56Z

134.76.70.252 at 14:25, 16 May 2013

2013-05-16T14:25:37Z

134.76.70.252 at 11:07, 14 May 2013

2013-05-14T11:07:29Z

134.76.70.252 at 13:42, 13 May 2013

2013-05-13T13:42:54Z

Awiechert at 09:28, 5 December 2012

2012-12-05T09:28:12Z

134.76.70.252 at 10:25, 19 September 2012

2012-09-19T10:25:35Z

134.76.70.252 at 15:22, 7 August 2012

2012-08-07T15:22:46Z

← Older revision		Revision as of 14:37, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' outer spore coat protein, spore associated transglutaminase <br/><br/>~~	+	{{#bank: Tgl}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''tgl''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yuxF, yugV ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| spore associated transglutaminase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| introduction of crosslinks in the spore coat protein [[GerQ]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270 tgl]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 28 kDa, 6.687~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 735 bp, 245 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[mcpB]]'', ''[[yuzH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:tgl_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:tgl_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GerE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigK regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU31270
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tgl.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10946]
−
−	~~=== Additional information===~~
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH<sub>3</sub> (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as [[GerQ]] (together with [[YabG]]) {{PubMed\|16751597}}
−
−	* '''Protein family:''' bacillus TGase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** outer spore coat, localization depends on [[CotE]] {{PubMed\|22171814}}
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P40746 P40746]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31270]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.2.13 2.3.2.13]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[tgl]]'' {{PubMed\|9274030}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 tgl] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigK]] {{PubMed\|15699190,12501297}}, [[SigE]] {{PubMed\|15383836}}
−
−	* '''Regulation:'''
−	** expressed late during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]]) {{PubMed\|15383836,15699190,12501297}}
−
−	* '''Regulatory mechanism:'''
−	** [[GerE]]: transcription repression {{PubMed\|15383836}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1S122 ( ''tgl''::''erm''), {{PubMed\|15317760}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S122&Search=1S122 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Adriano Henriques]], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>19797877 </pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>18249137, 22171814,12501297,16267299,11193401, 16751597 9274030 15699190 15383836 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 113: / Line 113: @@
 * '''Regulatory mechanism:'''
-** GerE: transcription repression {{PubMed|15383836}}
+** [[GerE]]: transcription repression {{PubMed|15383836}}
 * '''Additional information:'''

@@ Line 107: / Line 107: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 tgl] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigK]] {{PubMed|15699190,12501297}}, SigE
+* '''Sigma factor:''' [[SigK]] {{PubMed|15699190,12501297}}, [[SigE]] {{PubMed|15383836}}
 * '''Regulation:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' spore associated transglutaminase <br/><br/>
+* '''Description:''' outer spore coat protein, spore associated transglutaminase <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 45: / Line 41: @@
 = This gene is a member of the following [[regulons]] =
 {{SubtiWiki regulon|[[SigK regulon]]}}
@@ Line 62: / Line 60: @@
 === Additional information===
@@ Line 91: / Line 88: @@
 * '''[[Localization]]:'''
 === Database entries ===
@@ Line 109: / Line 107: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 tgl] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigK]] {{PubMed|15699190,12501297}}
+* '''Sigma factor:''' [[SigK]] {{PubMed|15699190,12501297}}, SigE
 * '''Regulation:'''
-** expressed late during sporulation in the mother cell ([[SigK]]) {{PubMed|15699190,12501297}}
+** expressed late during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]]) {{PubMed|15383836,15699190,12501297}}
 * '''Regulatory mechanism:'''
 * '''Additional information:'''
@@ Line 143: / Line 142: @@
 <pubmed>19797877 </pubmed>
 ==Original Publications==
-<pubmed>18249137,,12501297,16267299,11193401, 16751597 9274030 15699190</pubmed>
+<pubmed>18249137, 22171814,12501297,16267299,11193401, 16751597 9274030 15699190 15383836 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:tgl_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tgl_3212591_3213328_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:tgl_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:tgl_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || introduction of crosslinks in the spore coat protein [[GerQ]]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31270 tgl]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270 tgl]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.687
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15105&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:tgl_context.gif]]

@@ Line 20: / Line 20: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 735 bp, 245 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yugU]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]''
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15105&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

← Older revision		Revision as of 10:25, 19 September 2012
Line 121:		Line 121:

	* '''Mutant:'''		* '''Mutant:'''
		+	** 1S122 ( ''tgl''::''erm''), {{PubMed\|15317760}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S122&Search=1S122 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''