SspA - Revision history

134.76.70.252 at 09:30, 17 April 2014

2014-04-17T09:30:15Z

134.76.38.147 at 14:31, 2 April 2014

2014-04-02T14:31:10Z

Jstuelk at 16:35, 20 December 2013

2013-12-20T16:35:06Z

Fabian Commichau: /* Biological materials */

2013-07-12T07:31:41Z

‎Biological materials

Kgunka: /* Biological materials */

2013-07-11T13:48:03Z

‎Biological materials

Kgunka: /* Biological materials */

2013-07-11T13:47:44Z

‎Biological materials

134.76.70.252 at 14:18, 16 May 2013

2013-05-16T14:18:37Z

134.76.70.252 at 11:01, 14 May 2013

2013-05-14T11:01:02Z

134.76.70.252 at 13:36, 13 May 2013

2013-05-13T13:36:33Z

Jstuelk at 17:22, 16 October 2012

2012-10-16T17:22:39Z

← Older revision		Revision as of 09:30, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' small acid-soluble spore protein (major alpha-type SASP) <br/><br/>~~	+	{{#bank: SspA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''sspA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| small acid-soluble spore protein (major alpha-type SASP)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protection of spore DNA~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29570 sspA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 6 kDa, 4.731~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 207 bp, 69 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ytcI]]'', ''[[thiI]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29570 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:sspA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sspA_3025445_3025654_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:sspA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29570]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigG regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SpoVT regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29570
−
−	~~===Phenotypes of a mutant ===~~
−	* a ''[[sspA]]'' mutant is sensitive to ionizing radiation {{PubMed\|24123749}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29570&redirect=T BSU29570]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10786]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' alpha/beta-type SASP family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29570&redirect=T BSU29570]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2BZ0 2BZ0] (82% identity, 89% similarity)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P04831 P04831]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29570]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[sspA]]'' {{PubMed\|3009398}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sspA_3025445_3025654_-1 sspA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigG]] {{PubMed\|15699190}}
−
−	* '''Regulation:'''
−	** expressed late during sporulation in the forespore ([[SigG]], [[SpoVT]]) {{PubMed\|15699190,8755877}}
−
−	* '''Regulatory mechanism:'''
−	** [[SpoVT]]: transcription activation {{PubMed\|8755877}}
−
−	* '''Additional information:''' the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** BP137 (''[[sspA]]''::''cat'') available in [[Fabian Commichau]]'s lab
−	** 1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
−	** 1S111 (no resistance), {{PubMed\|3087950}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]
−	** 1S112 (no resistance), {{PubMed\|3125155}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S112&Search=1S112 BGSC]
−	** 1S113 (no resistance), {{PubMed\|3125155}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S113&Search=1S113 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>16707666,2456528,3009398,12884008,11092849,7608092,15458366, 19542328 15699190,8755877 23064347 3087950 3125155 24123749</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 54: / Line 54: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspA.html]
@@ Line 89: / Line 90: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2BZ0 2BZ0] (82% identity, 89% similarity)

@@ Line 51: / Line 51: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 59: / Line 60: @@
 === Additional information===
@@ Line 77: / Line 76: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 142: / Line 141: @@
 =References=
-<pubmed>16707666,2456528,3009398,12884008,11092849,7608092,15458366, 19542328 15699190,8755877 23064347 3087950 3125155</pubmed>
+<pubmed>16707666,2456528,3009398,12884008,11092849,7608092,15458366, 19542328 15699190,8755877 23064347 3087950 3125155 24123749</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 120: / Line 120: @@
 * '''Mutant:'''
-** BP137 (cat) available in [[Fabian Commichau]]'s lab
+** BP137 (''[[sspA]]''::''cat'') available in [[Fabian Commichau]]'s lab
 ** 1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
 ** 1S111 (no resistance), {{PubMed|3087950}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]

@@ Line 120: / Line 120: @@
 * '''Mutant:'''
-** BP137 (cat)available in [[Fabian Commichau]]'s lab
+** BP137 (cat) available in [[Fabian Commichau]]'s lab
 ** 1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
 ** 1S111 (no resistance), {{PubMed|3087950}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sspA_3025445_3025654_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sspA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sspA_3025445_3025654_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sspA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29570]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytcI]]'', ''[[thiI]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29570 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29570 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:sspA_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29570 sspA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29570 sspA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 4.731
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytcI]]'', ''[[thiI]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14935&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29570 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:sspA_context.gif]]

@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 124: / Line 120: @@
 * '''Mutant:'''
-** 1S109 (no resistance), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
+** 1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
 ** 1S111 (no resistance), {{PubMed|3087950}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]
 ** 1S112 (no resistance), {{PubMed|3125155}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S112&Search=1S112 BGSC]
@@ Line 145: / Line 141: @@
 =References=
-<pubmed>16707666,2456528,3009398,12884008,11092849,7608092,15458366, 19542328 15699190,8755877 </pubmed>
+<pubmed>16707666,2456528,3009398,12884008,11092849,7608092,15458366, 19542328 15699190,8755877 23064347 3087950 3125155</pubmed>
 [[Category:Protein-coding genes]]