SppA - Revision history

134.76.70.252 at 09:51, 17 April 2014

2014-04-17T09:51:38Z

134.76.70.252 at 11:44, 8 April 2014

2014-04-08T11:44:20Z

134.76.38.147 at 14:31, 2 April 2014

2014-04-02T14:31:03Z

Jstuelk at 14:26, 30 December 2013

2013-12-30T14:26:50Z

Jstuelk at 09:13, 19 November 2013

2013-11-19T09:13:49Z

Jstuelk at 18:10, 30 August 2013

2013-08-30T18:10:56Z

134.76.70.252 at 14:18, 16 May 2013

2013-05-16T14:18:28Z

134.76.70.252 at 11:00, 14 May 2013

2013-05-14T11:00:53Z

134.76.70.252 at 13:36, 13 May 2013

2013-05-13T13:36:22Z

134.76.70.252 at 14:56, 7 August 2012

2012-08-07T14:56:53Z

← Older revision		Revision as of 09:51, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' signal peptide peptidase required for efficient processing of pre-proteins, cleaves remnant signal peptides within the cellular membrane <br/><br/>~~	+	{{#bank: SppA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''sppA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yteI ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| signal peptide peptidase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| protein secretion~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29530 sppA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=SppA SppA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 36 kDa, 7.314~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1005 bp, 335 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yteJ]]'', ''[[ytdI]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29530 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29530 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29530 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:sppA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sppA_3020040_3021047_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:sppA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29530]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein secretion]]}},~~
−	~~{{SubtiWiki category\|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigW regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU29530
−
−	~~===Phenotypes of a mutant ===~~
−	* more sensitive to nisin {{PubMed\|23980836}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29530&redirect=T BSU29530]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sppA-yteJ.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13839]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** serine protease that functions to cleave the remnant signal peptides left behind after [[protein secretion]] and cleavage by signal peptidases
−
−	* '''Protein family:''' peptidase S49 family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** the protein forms a dome shaped octameric complex {{PubMed\|22472423}}
−
−	* '''[[Localization]]:'''
−	** cell membrane {{PubMed\|22472423}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29530&redirect=T BSU29530]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3RST 3RST] {{PubMed\|22472423}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34525 O34525]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29530]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[sppA]]-[[yteJ]]'' {{PubMed\|9987136}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sppA_3020040_3021047_-1 sppA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigW]] {{PubMed\|9987136,12207695}}
−
−	* '''Regulation:'''
−	** induced in response to cell wall stress ([[SigW]]) {{PubMed\|12207695}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** self-processes its own C-termini {{PubMed\|24228759}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>9987136,12207695 22472423 23980836 24228759 24228759 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29530 sppA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in  [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=SppA SppA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 7.314

@@ Line 57: / Line 57: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sppA-yteJ.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''	[http://www.rcsb.org/pdb/explore/explore.do?structureId=3RST 3RST] {{PubMed|22472423}}

@@ Line 1: / Line 1: @@
-* '''Description:''' signal peptide peptidase required for efficient processing of pre-proteins <br/><br/>
+* '''Description:''' signal peptide peptidase required for efficient processing of pre-proteins, cleaves remnant signal peptides within the cellular membrane <br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 80: / Line 80: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 111: / Line 111: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sppA_3020040_3021047_-1 sppA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigW]] {{PubMed|9987136,12207695}}
+* '''[[Sigma factor]]:''' [[SigW]] {{PubMed|9987136,12207695}}
 * '''Regulation:'''
@@ Line 141: / Line 141: @@
 =References=
-<pubmed>9987136,12207695 22472423 23980836 24228759 </pubmed>
+<pubmed>9987136,12207695 22472423 23980836 24228759 24228759 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 119: / Line 119: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 140: / Line 141: @@
 =References=
-<pubmed>9987136,12207695 22472423 23980836</pubmed>
+<pubmed>9987136,12207695 22472423 23980836 24228759 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 58: / Line 54: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 143: / Line 140: @@
 =References=
-<pubmed>9987136,12207695 22472423 </pubmed>
+<pubmed>9987136,12207695 22472423 23980836</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sppA_3020040_3021047_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sppA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sppA_3020040_3021047_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:sppA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29530]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yteJ]]'', ''[[ytdI]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29530 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29530 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29530 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29530 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29530 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29530 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:sppA_context.gif]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || protein secretion
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''