SecA - Revision history

Jstuelk: /* References */

2014-09-19T10:38:34Z

‎References

Jstuelk: /* References */

2014-06-22T16:57:21Z

‎References

Jstuelk: /* References */

2014-06-22T16:55:39Z

‎References

Jstuelk: /* References */

2014-06-22T16:54:20Z

‎References

Jstuelk: /* References */

2014-06-22T16:52:50Z

‎References

Jstuelk: /* References */

2014-06-22T16:52:18Z

‎References

Jstuelk at 06:10, 5 May 2014

2014-05-05T06:10:55Z

Jstuelk: /* References */

2014-05-05T06:09:58Z

‎References

134.76.70.252 at 14:14, 17 April 2014

2014-04-17T14:14:23Z

134.76.70.252 at 09:43, 17 April 2014

2014-04-17T09:43:24Z

← Older revision		Revision as of 10:38, 19 September 2014
Line 1:		Line 1:
−	* '''Description:~~''' preprotein translocase subunit (ATPase), required for membrane targeting of [[DivIVA]], motor protein that drives preprotein translocation through the [[SecY]]-[[SecE]]-[[SecG]] channel <br/><br/>~~	+	{{#bank: SecA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''secA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''div, div-341, ts-341 ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| preprotein translocase subunit (ATPase)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[protein secretion]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35300 secA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SecA SecA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=SecA SecA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 95 kDa, 5.34~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 2523 bp, 841 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[prfB]]'', ''[[yvyD]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35300 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35300 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35300 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:secA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=secA_3628310_3630835_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:secA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35300]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein secretion]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU35300
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35300&redirect=T BSU35300]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10741]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** ATP -> ADP + Pi + preprotein translocation
−	** required for membrane targeting of [[DivIVA]] {{PubMed\|24592260}}
−
−	* '''Protein family:''' SecA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:''' nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:''' Mg
−
−	* '''Effectors of protein activity:''' anionic phospholipids, preprotein, [[SecY]], signal peptides (even when added in trans) [http://www.ncbi.nlm.nih.gov/pubmed/19924216 PubMed]
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** ([[SecY]]-[[SecE]]-[[SecG]])2-[[SecA]]2 {{PubMed\|18923516}}
−	** [[SecA]]-[[Ffh]] {{PubMed\|9880811}}
−	** [[CsaA]]-[[SecA]] {{PubMed\|10816431}}
−	** [[DivIVA]]-[[SecA]] {{PubMed\|24592260}}
−
−	* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35300&redirect=T BSU35300]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure), [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM], [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3DL8 3DL8] (structure of the ([[SecY]]-[[SecE]]-[[SecG]])-[[SecA]] complex {{PubMed\|18923516}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P28366 P28366]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35300]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−	* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[secA]]-[[prfB]]'' {{PubMed\|9882663}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=secA_3628310_3630835_-1 secA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|9882663}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 945 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 2829 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 411 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 395 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 564 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant: ''Pspac-[[secA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/12218047 PubMed]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 8497195 8440733 7706219 7642557 </pubmed>
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 151: / Line 151: @@
 =References=
-<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 8497195 8440733 </pubmed>
+<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 8497195 8440733 7706219 7642557 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 151: / Line 151: @@
 =References=
-<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 </pubmed>
+<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 8497195 8440733 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 151: / Line 151: @@
 =References=
-<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 </pubmed>
+<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 7894702 10074074 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 151: / Line 151: @@
 =References=
-<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 </pubmed>
+<pubmed>19933328, 19924216, 18923516,12873133, 12242434,1385592,10816431,19850053,9880811, 12218047 17981983 9882663 23484952 20574771 23167435 23794293 23852076 24592260 24786965 11021932 7851746 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 06:10, 5 May 2014
Line 1:		Line 1:
−	* '''Description:''' preprotein translocase subunit (ATPase), required for membrane targeting of [[DivIVA]] <br/><br/>	+	* '''Description:''' preprotein translocase subunit (ATPase), required for membrane targeting of [[DivIVA]], motor protein that drives preprotein translocation through the [[SecY]]-[[SecE]]-[[SecG]] channel <br/><br/>

	{\| align="right" border="1" cellpadding="2"		{\| align="right" border="1" cellpadding="2"

@@ Line 129: / Line 129: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 945 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2829 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant: ''Pspac-[[secA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/12218047 PubMed]

@@ Line 127: / Line 127: @@
 * '''Additional information:'''
 ** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
 =Biological materials =