SdhA - Revision history

134.76.70.252 at 14:14, 17 April 2014

2014-04-17T14:14:19Z

134.76.70.252 at 09:55, 17 April 2014

2014-04-17T09:55:35Z

134.76.38.147 at 14:26, 2 April 2014

2014-04-02T14:26:41Z

134.76.70.252 at 11:23, 7 January 2014

2014-01-07T11:23:55Z

134.76.70.252 at 09:04, 12 November 2013

2013-11-12T09:04:04Z

Jstuelk: /* Biological materials */

2013-08-13T14:12:56Z

‎Biological materials

Jstuelk: /* Biological materials */

2013-08-13T14:12:20Z

‎Biological materials

Aklewing: /* Biological materials */

2013-08-13T12:08:36Z

‎Biological materials

Jstuelk: /* References */

2013-08-12T09:40:18Z

‎References

Jstuelk at 18:04, 11 August 2013

2013-08-11T18:04:17Z

← Older revision		Revision as of 14:14, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' succinate dehydrogenase (flavoprotein subunit) <br/><br/>~~	+	{{#bank: SdhA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''sdhA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''citF ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| succinate dehydrogenase (flavoprotein subunit)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| TCA cycle~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28440 sdhA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SdhA SdhA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sdhA sdhA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 65 kDa, 5.714~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1758 bp, 586 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[sdhB]], [[sdhC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28440 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:sdhA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sdhA_2906335_2908095_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:sdhA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28440]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[carbon core metabolism]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[FsrA regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU28440
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28440&redirect=T BSU28440]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sdhCAB.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10352]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Succinate + acceptor = fumarate + reduced acceptor (according to Swiss-Prot)
−
−	* '''Protein family:''' FRD/SDH subfamily (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:''' phosphorylated [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
−
−	* '''Cofactor(s):''' Fe
−
−	* '''Effectors of protein activity:'''
−	** Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed]
−	** Activated by Cytochrome b558 [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[SdhA]]-[[SdhB]] (as part of the [[SdhC]]-[[SdhB]]-[[SdhA]] complex)
−	** [[FloT]]-[[SdhA]] {{PubMed\|23651456}}
−	** part of a [[Respiratory complexes and supercomplexes\|respiratory supercomplex]] {{PubMed\|23880299}}
−
−	* '''[[Localization]]:'''
−	** attached to the membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28440&redirect=T BSU28440]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NEK 1NEK] (''E. coli'')
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P08065 P08065]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28440]
−
−	* '''E.C. number:''' 1.3.99.1
−
−	~~=== Additional information===~~
−	* This enzyme is a membrane-bound trimer [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
−	** One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
−	** Another subunit is the flavoprotein one, required for FAD usage [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
−	** The other subunit has an iron-sulphur domain necessary for the catalytic activity [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
−	* extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[sdhC]]''-''[[sdhA]]''-''[[sdhB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/3036777 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sdhA_2906335_2908095_-1 sdhA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2495271 PubMed]
−
−	* '''Regulation:'''
−	** constitutive
−	** part of the iron sparing response ([[FsrA]]) {{PubMed\|22389480}}
−
−	* '''Regulatory mechanism:'''
−	** [[FsrA]]: translation inhibition {{PubMed\|22389480}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1389 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 14010 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP743 (''[[sdhC]]-[[sdhA]]'', cat), available in [[Jörg Stülke]]'s lab
−	** GP792 (''[[sdhC]]-[[sdhA]]-[[sdhB]]''::''phleo''), available in [[Jörg Stülke]]'s lab
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 11803018 </pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680 18763711 17726680 16493705 3910107 6799760 12560550 23651456 23880299</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 09:55, 17 April 2014
Line 137:		Line 137:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1389 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 14010 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 60: / Line 60: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sdhCAB.html]
@@ Line 103: / Line 104: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NEK 1NEK] (''E. coli'')

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SdhA SdhA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sdhA sdhA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 5.714

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28440 sdhA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SdhA SdhA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SdhA SdhA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''

@@ Line 148: / Line 148: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
 * '''Antibody:'''

@@ Line 138: / Line 138: @@
 =Biological materials =
-* '''Mutant:''' GP743 (''sdhCA'', cat), available in [[Stülke]] lab
+* '''Mutant:'''
-** GP792 (''sdhCAB''::''phleo''), available in [[Jörg Stülke]]'s lab
+** GP743 (''[[sdhC]]-[[sdhA]]'', cat), available in [[Jörg Stülke]]'s lab
 * '''Expression vector:'''

← Older revision		Revision as of 12:08, 13 August 2013
Line 139:		Line 139:

	* '''Mutant:''' GP743 (''sdhCA'', cat), available in [[Stülke]] lab		* '''Mutant:''' GP743 (''sdhCA'', cat), available in [[Stülke]] lab
		+	** GP792 (''sdhCAB''::''phleo''), available in [[Jörg Stülke]]'s lab

	* '''Expression vector:'''		* '''Expression vector:'''

@@ Line 155: / Line 155: @@
 =References=
+== Reviews ==
 <pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680 18763711 17726680 16493705 3910107 6799760 12560550  23651456 23880299</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 97: / Line 97: @@
 ** [[SdhA]]-[[SdhB]] (as part of the [[SdhC]]-[[SdhB]]-[[SdhA]] complex)
 ** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
-* '''[[Localization]]:''' attached to the membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
+* '''[[Localization]]:'''
 === Database entries ===
@@ Line 154: / Line 156: @@
 =References=
-<pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680 18763711 17726680 16493705 3910107 6799760 12560550  23651456</pubmed>
+<pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680 18763711 17726680 16493705 3910107 6799760 12560550  23651456 23880299</pubmed>
 [[Category:Protein-coding genes]]