SasB - Revision history

Jstuelk: /* References */

2015-02-10T17:56:38Z

‎References

Jstuelk: /* Phenotypes of a mutant */

2015-02-10T17:55:45Z

‎Phenotypes of a mutant

134.76.70.252 at 11:25, 17 April 2014

2014-04-17T11:25:44Z

Jstuelk: /* Expression and regulation */

2014-02-06T16:43:08Z

‎Expression and regulation

Jstuelk at 16:40, 6 February 2014

2014-02-06T16:40:54Z

Jstuelk: /* Expression and regulation */

2014-02-06T16:25:45Z

‎Expression and regulation

Jstuelk: /* Basic information/ Evolution */

2014-02-06T16:00:05Z

‎Basic information/ Evolution

Jstuelk at 15:59, 6 February 2014

2014-02-06T15:59:33Z

Jstuelk: Jstuelk moved page YjbM to SasB: 24489751

2014-02-06T15:52:14Z

Jstuelk moved page YjbM to SasB: 24489751

Jstuelk at 11:46, 22 December 2013

2013-12-22T11:46:46Z

← Older revision		Revision as of 17:56, 10 February 2015
Line 1:		Line 1:
−	* '''Description:''' (p)ppGpp synthetase <br/><br/>	+	{{#bank: SasB}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''sasB''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yjbM ''~~
−	\|-
−	~~\|style="background:~~#~~ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background~~:~~#ABCDEF;" align="center"\| '''Product''' \|\| (p)ppGpp synthetase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| ppGpp synthesis independent from stringent response~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11600 sasB]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 24 kDa, 6.708~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 633 bp, 211 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yjbL]]'', ''[[nadF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11600 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yjbM_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 Expression at a glance]'''   {{PubMed\|22383849}~~}~~<br/>[[Image:yjbM_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11600]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[metabolism of signalling nucleotides]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU11600
−
−	~~===Phenotypes of a mutant ===~~
−	* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed\|24163341}}
−	* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant acquires suppressor mutations in ''[[guaA]], [[guaB]], [[gmk]]'' or inactivation of ''[[codY]]'' {{PubMed\|24682323,24163341}}
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13142]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):''' [[SasA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:''' single ppGpp synthetase domain {{PubMed\|24163341}}
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O31611 O31611]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11600]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[yjbL]]-[[sasB]]-[[nadF]]-[[yjbO]]'' {{PubMed\|18067544}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 sasB] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:''' expressed during exponential growth
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 169 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>22950019 18670626 19447912 18067544 24163341 24489751 </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 50: / Line 50: @@
 ===Phenotypes of a mutant ===
 * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
 === Database entries ===

← Older revision		Revision as of 11:25, 17 April 2014
Line 110:		Line 110:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 169 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 101: / Line 101: @@
 * '''Operon:''' ''[[yjbL]]-[[sasB]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}}
-* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 yjbM] {{PubMed|22383849}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 sasB] {{PubMed|22383849}}
 * '''[[Sigma factor]]:'''

@@ Line 3: / Line 3: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Gene name'''
-|''yjbM''
+|''sasB''
 |-
-|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjbM ''
 |-
 |style="background:#ABCDEF;" align="center"| '''Essential''' || no

@@ Line 99: / Line 99: @@
 =Expression and regulation=
-* '''Operon:''' ''[[yjbL]]-[[yjbM]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}}
+* '''Operon:''' ''[[yjbL]]-[[sasB]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 yjbM] {{PubMed|22383849}}

@@ Line 67: / Line 67: @@
 * '''Protein family:'''
-* '''Paralogous protein(s):''' [[YwaC]]
+* '''Paralogous protein(s):''' [[SasA]]
 === Extended information on the protein ===

@@ Line 13: / Line 13: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || ppGpp synthesis independent from stringent response
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11600 yjbM]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11600 sasB]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 6.708
@@ Line 49: / Line 49: @@
 ===Phenotypes of a mutant ===
-* a ''[[relA]] [[yjbM]] [[ywaC]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
+* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
 === Database entries ===
@@ Line 58: / Line 58: @@
 === Additional information===
 =The protein=
@@ Line 131: / Line 130: @@
 =References=
-<pubmed>22950019 18670626 19447912 18067544 24163341</pubmed>
+<pubmed>22950019 18670626 19447912 18067544 24163341 24489751 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision	Revision as of 15:52, 6 February 2014
(No difference)

@@ Line 49: / Line 49: @@
 ===Phenotypes of a mutant ===
 === Database entries ===
@@ Line 73: / Line 74: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:''' single ppGpp synthetase domain {{PubMed|24163341}}
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 103: / Line 104: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yjbM_1237006_1237641_1 yjbM] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:''' expressed during exponential growth
@@ Line 130: / Line 131: @@
 =References=
-'''Additional publications:''' {{PubMed|22950019}}
+<pubmed>22950019 18670626 19447912 18067544 24163341</pubmed>
 [[Category:Protein-coding genes]]