RsbT - Revision history

134.76.70.252 at 09:33, 17 April 2014

2014-04-17T09:33:19Z

134.76.38.147 at 13:02, 2 April 2014

2014-04-02T13:02:30Z

Jstuelk at 17:26, 8 March 2014

2014-03-08T17:26:35Z

134.76.70.252 at 10:12, 7 January 2014

2014-01-07T10:12:10Z

134.76.70.252 at 15:14, 11 November 2013

2013-11-11T15:14:55Z

134.76.70.252 at 12:36, 16 May 2013

2013-05-16T12:36:36Z

134.76.70.252 at 09:32, 14 May 2013

2013-05-14T09:32:03Z

134.76.70.252 at 12:11, 13 May 2013

2013-05-13T12:11:45Z

Jstuelk at 13:46, 19 February 2013

2013-02-19T13:46:20Z

Jstuelk at 11:21, 15 February 2013

2013-02-15T11:21:56Z

← Older revision		Revision as of 09:33, 17 April 2014
Line 1:		Line 1:
−	* '''Description:''' PP2C activator, [[protein kinases and phosphatases\|protein serine kinase]], phosphorylates [[RsbS]] and [[RsbR]], part of the [[stressosome]] <br/><br/>	+	{{#bank: RsbT}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''rsbT''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''ycxT ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| PP2C activator, [[protein kinases and phosphatases\|protein serine kinase]]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| control of [[SigB]] activity~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04690 rsbT]
−	\|-
−	~~\|colspan="2" style="background:~~#~~FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]'''~~: ~~[http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RsbT~~ RsbT]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rsbT rsbT]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 14 kDa, 6.587~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 399 bp, 133 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[rsbS]]'', ''[[rsbU]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04690 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:rsbT_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbT_520606_521007_1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:rsbT_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04690]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[protein modification]]}},~~
−	~~{{SubtiWiki category\|[[sigma factors and their control]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU04690
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04690&redirect=T BSU04690]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsbRSTUVW-sigB-rsbX.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11092]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' phosphorylation of [[RsbR]], [[RsbRB]], [[RsbRC]], and [[RsbRD]] {{PubMed\|21362065}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−	** phosphorylated [[RsbR]] activates the kinase activity of [[RsbT]] {{PubMed\|23320651}}
−	** activity is stimulated by light in a [[YtvA]]-dependent manner {{PubMed\|23416074}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[RsbS]]-[[RsbT]] {{PubMed\|16321960}}
−	** [[RsbR]]-[[RsbT]] {{PubMed\|21362065}}
−	** [[RsbRB]]-[[RsbT]] {{PubMed\|21362065}}
−	** [[RsbRC]]-[[RsbT]] {{PubMed\|21362065}}
−	** [[RsbRD]]-[[RsbT]] {{PubMed\|21362065}}
−	** [[RsbT]]-[[RsbU]]
−
−	** component of the [[stressosome]]
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04690&redirect=T BSU04690]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3VY9 3VY9] (complete stressosome)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P42411 P42411]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04690]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[rsbR]]-[[rsbS]]-[[rsbT]]-[[rsbU]]-[[rsbV]]-[[rsbW]]-[[sigB]]-[[rsbX]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbT_520606_521007_1 rsbT] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
−
−	* '''Regulation:''' constitutively expressed {{PubMed\|20019076}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** [[RsbT]] is synthesized at the same rate as [[RsbRA]] and [[RsbS]], however, the [[RsbT]] level in growing ''B. subtilis'' is only 10% that of [[RsbRA]], suggesting that the protein is unstable {{PubMed\|20019076}}.
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	* [[Bill Haldenwang]], San Antonio, USA
−	* [[Chet Price]], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
−	* [[Rick Lewis]], Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/r.lewis homepage]
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed>19704888 16319496 20658979 </pubmed>~~
−	~~==Original Articles==~~
−	<pubmed>8682789,8002610,8682769,9658013,17303566, 9786195,15090521,10781545, 15583165, 8824586, 10329124,16321960,8808936,15312768,11244072,15342582,20019076, 12499568, 12950928, 16321960, 8955331, 18832644 24599254 23407164,23320651,21362065 23416074 21979936</pubmed>
−	~~[[Category:Protein-coding genes]]~~

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsbRSTUVW-sigB-rsbX.html]
@@ Line 101: / Line 102: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3VY9 3VY9] (complete stressosome)

@@ Line 78: / Line 78: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 152: / Line 152: @@
 ==Reviews==
 <pubmed>19704888 16319496  20658979 </pubmed>
 ==Original Articles==
-'''Additional publications:''' {{PubMed|23407164,23320651,21362065}}
+<pubmed>8682789,8002610,8682769,9658013,17303566, 9786195,15090521,10781545, 15583165, 8824586, 10329124,16321960,8808936,15312768,11244072,15342582,20019076, 12499568, 12950928, 16321960, 8955331,  18832644 24599254 23407164,23320651,21362065 23416074 21979936</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 17: / Line 17: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RsbT RsbT]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rsbT rsbT]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 6.587

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04690 rsbT]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RsbT RsbT]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RsbT RsbT]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''

@@ Line 30: / Line 30: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbT_520606_521007_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rsbT_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbT_520606_521007_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rsbT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04690]]
 |-
 |}

@@ Line 25: / Line 25: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsbS]]'', ''[[rsbU]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04690 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04690 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:rsbT_context.gif]]

@@ Line 86: / Line 86: @@
 * '''Effectors of protein activity:'''
 ** phosphorylated [[RsbR]] activates the kinase activity of [[RsbT]] {{PubMed|23320651}}
 * '''[[SubtInteract|Interactions]]:'''
@@ Line 94: / Line 95: @@
 ** [[RsbRD]]-[[RsbT]] {{PubMed|21362065}}
 ** [[RsbT]]-[[RsbU]]
 ** component of the [[stressosome]]
@@ Line 116: / Line 118: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsbT_520606_521007_1 rsbT] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
+* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8002610 PubMed]
 * '''Regulation:''' constitutively expressed {{PubMed|20019076}}
@@ Line 153: / Line 155: @@
 ==Original Articles==
 '''Additional publications:''' {{PubMed|23407164,23320651,21362065}}
   <big>''Locke JC, Young JW, Fontes M, Hernández Jiménez MJ, Elowitz MB''  </big>
   <big>'''Stochastic pulse regulation in bacterial stress response.''' </big>