RpsJ - Revision history

Jstuelk: /* Extended information on the protein */

2015-02-01T17:22:35Z

‎Extended information on the protein

Jstuelk: /* Original publications */

2015-02-01T17:21:43Z

‎Original publications

Jstuelk: /* Biological materials */

2014-12-11T17:33:56Z

‎Biological materials

Fabian Commichau: /* Biological materials */

2014-12-08T14:36:46Z

‎Biological materials

Fabian Commichau: /* Biological materials */

2014-12-08T14:34:38Z

‎Biological materials

134.76.70.252 at 09:32, 17 April 2014

2014-04-17T09:32:12Z

134.76.38.147 at 12:49, 2 April 2014

2014-04-02T12:49:26Z

134.76.70.252 at 15:00, 11 November 2013

2013-11-11T15:00:59Z

Jstuelk at 15:06, 14 June 2013

2013-06-14T15:06:07Z

134.76.70.252 at 12:22, 16 May 2013

2013-05-16T12:22:31Z

← Older revision		Revision as of 17:22, 1 February 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[ribosomal protein]] <br/><br/>~~	+	{{#bank: RpsJ}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''rpsJ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''tetA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes {{PubMed\|23002217,12682299}}~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[ribosomal protein]] S10 (BS13)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[translation]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01150 rpsJ]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RpsJ RpsJ]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 11 kDa, 10.207~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 306 bp, 102 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ybaC]]'', ''[[rplC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01150 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01150 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01150 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:rpsJ_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsJ_135364_135672_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:rpsJ_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01150]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[translation]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[universally conserved proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[stringent response]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU01150
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential {{PubMed\|23002217,12682299}}~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01150&redirect=T BSU01150]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG19008]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' [[ribosomal protein]] S10P family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** located near the aminoacyl-tRNA site of the 30S ribosomal subunit {{PubMed\|23759864}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01150&redirect=T BSU01150]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P21471 P21471]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01150]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[rpsJ]]-[[rplC]]-[[rplD]]-[[rplW]]-[[rplB]]-[[rpsS]]-[[rplV]]-[[rpsC]]-[[rplP]]-[[rpmC]]-[[rpsQ]]-[[rplN]]-[[rplX]]-[[rplE]]-[[rpsN]]-[[rpsH]]-[[rplF]]-[[rplR]]-[[rpsE]]-[[rpmD]]-[[rplO]]'' {{PubMed\|9371452}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsJ_135364_135672_1 rpsJ] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−	** [[SigA]] two promoters, about 140bp and 200bp upstream of the start codon {{PubMed\|9371452}}
−
−	* '''Regulation:'''
−	** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed\|11948165}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 8159 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 13952 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−	** pGP605 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab
−	** pGP2201 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
−	** pGP2439 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
−	** pGP2472 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab
−	** pGP2473 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15621428 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed> 19653700, 9371452 11948165 8635744 23759864 23002217 11007480</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 153: / Line 153: @@
 <pubmed> 15621428 </pubmed>
 ==Original publications==
-<pubmed> 19653700, 9371452 11948165 8635744 23759864 23002217< /pubmed>
+<pubmed> 19653700, 9371452 11948165 8635744 23759864 23002217 11007480</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 133: / Line 133: @@
 * '''lacZ fusion:'''
-** pGP605 (in [[pAC6]]), available  in [[Stülke]] lab
+** pGP605 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab
-** pGP2201 (in [[pAC7]]), available  in [[Stülke]] lab
+** pGP2201 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
-** pGP2439 (in [[pAC7]]), available  in [[Stülke]] lab
+** pGP2439 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab
-** pGP2472 (in [[pAC5]]), available  in [[Stülke]] lab
+** pGP2472 (in [[pAC5]]), available in [[Jörg Stülke]]'s lab
-** pGP2473 (in [[pAC6]]), available  in [[Stülke]] lab
+** pGP2473 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''

@@ Line 136: / Line 136: @@
 ** pGP2201 (in [[pAC7]]), available  in [[Stülke]] lab
 ** pGP2439 (in [[pAC7]]), available  in [[Stülke]] lab
 * '''GFP fusion:'''

@@ Line 135: / Line 135: @@
 ** pGP605 (in [[pAC6]]), available  in [[Stülke]] lab
 ** pGP2201 (in [[pAC7]]), available  in [[Stülke]] lab
 * '''GFP fusion:'''

← Older revision		Revision as of 09:32, 17 April 2014
Line 123:		Line 123:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 8159 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 13952 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 96: / Line 97: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01150 rpsJ]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RpsJ RpsJ]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RpsJ RpsJ]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 10.207

@@ Line 93: / Line 93: @@
 * '''[[Localization]]:'''
 === Database entries ===
@@ Line 111: / Line 112: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsJ_135364_135672_1 rpsJ] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 ** [[SigA]] two promoters, about 140bp and 200bp upstream of the start codon {{PubMed|9371452}}
@@ Line 145: / Line 146: @@
 <pubmed> 15621428 </pubmed>
 ==Original publications==
-'''Additional publications:''' {{PubMed|23002217}}
+<pubmed> 19653700, 9371452 11948165 8635744 23759864 23002217< /pubmed>
 [[Category:Protein-coding genes]]

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsJ_135364_135672_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rpsJ_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpsJ_135364_135672_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rpsJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01150]]
 |-
 |}