RpoA - Revision history

Jstuelk at 16:28, 30 August 2015

2015-08-30T16:28:45Z

Jstuelk: /* Original publications */

2015-07-13T14:00:14Z

‎Original publications

134.76.70.252 at 14:14, 17 April 2014

2014-04-17T14:14:00Z

134.76.70.252 at 09:45, 17 April 2014

2014-04-17T09:45:51Z

134.76.38.147 at 12:50, 2 April 2014

2014-04-02T12:50:38Z

Jstuelk at 16:04, 5 March 2014

2014-03-05T16:04:11Z

134.76.70.252 at 15:02, 11 November 2013

2013-11-11T15:02:28Z

134.76.70.252 at 12:23, 16 May 2013

2013-05-16T12:23:58Z

134.76.70.252 at 09:17, 14 May 2013

2013-05-14T09:17:56Z

134.76.70.252 at 11:59, 13 May 2013

2013-05-13T11:59:35Z

← Older revision		Revision as of 16:28, 30 August 2015
Line 1:		Line 1:
−	* '''Description:~~''' [[RNA polymerase]] alpha subunit <br/><br/>~~	+	{{#bank: RpoA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''rpoA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[RNA polymerase]] alpha subunit~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[transcription]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01430 rpoA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RpoA RpoA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 34 kDa, 4.593~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 942 bp, 314 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[rpsK]]'', ''[[rplQ]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01430 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:rpoA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoA_148931_149875_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:rpoA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01430]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[transcription]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~[[most abundant proteins]]~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[stringent response]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU01430
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01430&redirect=T BSU01430]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10732]
−
−	~~=== Additional information===~~
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)
−
−	* '''Protein family:''' [[RNA polymerase]] alpha chain family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[RpoA]]-[[RpoB]]-[[RpoC]] {{PubMed\|10499798}}
−	** [[CcpN]]-[[RpoA]] {{PubMed\|19726675}}
−	** [[RpoA]] (C-terminal domain) -[[Spx]] {{PubMed\|12642660,22307755}}
−	** [[MgsR]]-[[RpoA]] {{PubMed\|18643936}}
−	** [[CcpA]]-[[RpoA]] {{PubMed\|22512862}}
−	** [[RpoA]]-[[CodY]] {{PubMed\|22512862}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01430&redirect=T BSU01430]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Z3E 1Z3E] (C-terminal domain, complex with Spx)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P20429 P20429]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01430]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.6 2.7.7.6]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoA_148931_149875_1 rpoA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:'''
−
−	* '''Regulation:'''
−	** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed\|11948165}}
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 3504 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 11832 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 27116 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 13650 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 25767 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 22210308 </pubmed>~~
−	~~==Original publications==~~
−	~~<pubmed>20084284 19726675 19580872 ,3093467,2496109,8635744,10675340,12486038,18643936, 12642660, 16249335, 16740936 15378759 26154296 22512862,20817769,22307755</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 154: / Line 154: @@
 <pubmed> 22210308 </pubmed>
 ==Original publications==
-<pubmed>20084284 19726675 19580872 ,3093467,2496109,8635744,10675340,12486038,18643936, 12642660, 16249335, 16740936 15378759 22512862,20817769,22307755</pubmed>
+<pubmed>20084284 19726675 19580872 ,3093467,2496109,8635744,10675340,12486038,18643936, 12642660, 16249335, 16740936 15378759 26154296 22512862,20817769,22307755</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 129: / Line 129: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 3504 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 11832 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''

@@ Line 127: / Line 127: @@
 * '''Additional information:'''
 ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 =Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 99: / Line 100: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Z3E 1Z3E] (C-terminal domain, complex with Spx)

@@ Line 41: / Line 41: @@
 = [[Categories]] containing this gene/protein =
 {{SubtiWiki category|[[transcription]]}},
-{{SubtiWiki category|[[essential genes]]}}
+{{SubtiWiki category|[[essential genes]]}},
 = This gene is a member of the following [[regulons]] =
@@ Line 79: / Line 80: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 115: / Line 116: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoA_148931_149875_1 rpoA] {{PubMed|22383849}}
-* '''Sigma factor:'''
+* '''[[Sigma factor]]:'''
 * '''Regulation:'''
@@ Line 123: / Line 124: @@
 * '''Additional information:'''
 =Biological materials =
@@ Line 146: / Line 148: @@
 <pubmed> 22210308 </pubmed>
 ==Original publications==
-'''Additional publications:''' {{PubMed|22512862,20817769,22307755}}
+<pubmed>20084284 19726675 19580872 ,3093467,2496109,8635744,10675340,12486038,18643936, 12642660, 16249335, 16740936 15378759 22512862,20817769,22307755</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01430 rpoA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RpoA RpoA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RpoA RpoA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.593

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoA_148931_149875_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rpoA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoA_148931_149875_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:rpoA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01430]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpsK]]'', ''[[rplQ]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01430 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01430 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:rpoA_context.gif]]