RocG - Revision history

Jstuelk: /* Biological materials */

2015-05-26T10:43:17Z

‎Biological materials

Show changes

Jstuelk: /* Expression of rocG */

2015-04-17T16:09:15Z

‎Expression of rocG

Jstuelk: /* Expression of rocG */

2015-03-18T17:18:31Z

‎Expression of rocG

Jstuelk: /* Enzymatic activity of RocG */

2015-03-03T12:58:00Z

‎Enzymatic activity of RocG

134.76.70.252 at 10:09, 17 April 2014

2014-04-17T10:09:53Z

134.76.38.147 at 15:06, 2 April 2014

2014-04-02T15:06:24Z

Jstuelk: /* Additional publications */

2014-01-30T09:32:04Z

‎Additional publications

134.76.70.252 at 11:55, 7 January 2014

2014-01-07T11:55:47Z

134.76.70.252 at 09:36, 12 November 2013

2013-11-12T09:36:53Z

Jstuelk: /* Biological materials */

2013-07-21T16:38:53Z

‎Biological materials

@@ Line 180: / Line 180: @@
 <pubmed>15150225,17994626 ,17608797 17183217 20630473   </pubmed>
 ==Expression of ''rocG''==
-<pubmed>12634342,15150224 10468601 9829940 22900538,20817675 25777015</pubmed>
+<pubmed>12634342,15150224 10468601 9829940 22900538,20817675 25777015 25880922</pubmed>
 ==Structural analysis of glutamate dehydrogenase==

@@ Line 180: / Line 180: @@
 <pubmed>15150225,17994626 ,17608797 17183217 20630473   </pubmed>
 ==Expression of ''rocG''==
-<pubmed>12634342,15150224 10468601 9829940 22900538,20817675</pubmed>
+<pubmed>12634342,15150224 10468601 9829940 22900538,20817675 25777015</pubmed>
 ==Structural analysis of glutamate dehydrogenase==
 <pubmed>8263917 20630473 </pubmed>

@@ Line 175: / Line 175: @@
 ==Enzymatic activity of RocG==
-<pubmed>18603778,16244435 16195607 ,18326565, 9829940 20630473 21965396 </pubmed>
+<pubmed>18603778,16244435 16195607 ,18326565, 9829940 20630473 21965396 25711804</pubmed>
 ==Function in the control of [[GltC]] activity==
 <pubmed>15150225,17994626 ,17608797 17183217 20630473   </pubmed>

@@ Line 138: / Line 138: @@
 * '''Additional information:'''
 Activation by [[RocR]] requires binding of [[RocR]] to a downstream element {{PubMed|12634342}}
 =Biological materials =

@@ Line 66: / Line 66: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocG.html]
@@ Line 105: / Line 106: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K92 3K92] (super-repressor mutant that is capable of constitutive inactivation of [[GltC]], E93K mutation) {{PubMed|20630473}}

@@ Line 183: / Line 183: @@
 ==Additional publications==
-<pubmed>23338837 23419162 22178969</pubmed>
+<pubmed>23338837 23419162 22178969 24473333 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RocG RocG]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rocG rocG]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46.2 kDa, 6.28

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37790 rocG]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RocG RocG]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RocG RocG]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''

@@ Line 139: / Line 139: @@
 =Biological materials =
-* '''Mutant:''' GP747 (spc), GP726 (aphA3), GP810 (del tet), GP1157 (cat) all available in [[Stülke]] lab
+* '''Mutant:''' GP747 (spc), GP726 (aphA3), GP810 (del tet), GP1157 (cat) all available in [[Jörg Stülke]]'s lab
 * '''Expression vector:'''
-** expression of native ''rocG'' in ''B. subtilis'': pGP529 (in [[pBQ200]]), available in [[Stülke]] lab
+** expression of native ''rocG'' in ''B. subtilis'': pGP529 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab {{PubMed|18326565}}
-** for purification of RocG from ''E. coli'' carrying an N-terminal Strep-tag: pGP902 (in [[pGP172]]), a series of ''rocG'' variants is also available in [[pGP172]], available in [[Stülke]] lab
+** for purification of RocG from ''E. coli'' carrying an N-terminal Strep-tag: pGP902 (in [[pGP172]]), a series of ''rocG'' variants is also available in [[pGP172]], available in [[Jörg Stülke]]'s lab
-** for expression/ purification from ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pWH844]]: pGP860, available in [[Stülke]] lab
+** for expression/ purification from ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pWH844]]: pGP860, available in [[Jörg Stülke]]'s lab
-** purification from ''B. subtilis'' with an N-terminal Strep-tag, for [[SPINE]], (in [[pGP380]]): pGP1709, available in [[Stülke]] lab
+** purification from ''B. subtilis'' with an N-terminal Strep-tag, for [[SPINE]], (in [[pGP380]]): pGP1709, available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
@@ Line 151: / Line 151: @@
 * '''GFP fusion:'''
-* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
-* '''Antibody:''' available in [[Stülke]] lab
+* '''Antibody:''' available in [[Jörg Stülke]]'s lab
 =Labs working on this gene/protein=