RecU - Revision history

Jstuelk: /* Original publications */

2015-08-10T11:03:52Z

‎Original publications

Jstuelk at 09:15, 7 May 2015

2015-05-07T09:15:11Z

Jstuelk: /* Original publications */

2014-05-06T07:34:43Z

‎Original publications

Jstuelk at 16:47, 5 May 2014

2014-05-05T16:47:38Z

Jstuelk at 10:30, 5 May 2014

2014-05-05T10:30:50Z

Jstuelk at 10:27, 5 May 2014

2014-05-05T10:27:46Z

Jstuelk at 10:27, 5 May 2014

2014-05-05T10:27:05Z

134.76.38.147 at 14:02, 2 April 2014

2014-04-02T14:02:18Z

Jstuelk at 17:56, 14 December 2013

2013-12-14T17:56:09Z

134.76.70.252 at 08:42, 12 November 2013

2013-11-12T08:42:04Z

← Older revision		Revision as of 11:03, 10 August 2015
Line 1:		Line 1:
−	* '''Description:''' Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities, equivalent to ''E. coli'' RecU~~, required for efficient survival and replication restart after replication-transcription conflicts<br/><br/>~~	+	{{#bank: RecU}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''recU''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''recG, prfA, yppB, jopB ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| Holliday junction resolvase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[DNA repair/ recombination]] and chromosome segregation~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310 recU]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecU RecU]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 23 kDa, 9.511~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 618 bp, 206 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yppC]]'', ''[[ponA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22310 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:recU_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recU_2340802_2341422_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:recU_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[DNA repair/ recombination]]}},~~
−	~~{{SubtiWiki category\|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[SigM regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU22310
−
−	~~===Phenotypes of a mutant ===~~
−	* the inactivations of ''[[recU]]'' and ''[[ruvA]]-[[ruvB]]'' are synthetically lethal {{PubMed\|24770420}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22310&redirect=T BSU22310]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/recU-ponA.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10953]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−	** binds Holliday junctions with high affinity {{PubMed\|24770420}}
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−	** [[RecU]]-mediated Holliday junction resolution is stimulated by [[RuvB]]-ATP {{PubMed\|24770420}}
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** form dimers {{PubMed\|21600217}}
−	** [[RecA]]-[[RecU]] {{PubMed\|16024744}}
−	** [[RecU]]-[[RuvB]] {{PubMed\|24770420,21600217}}
−	** formation of a ternary complex [[RecU]]-[[RuvB]]-Holliday junction DNA {{PubMed\|24770420}}
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22310&redirect=T BSU22310]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZP7 1ZP7]
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39792 P39792]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22310]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[recU]]-[[ponA]]'' {{PubMed\|7814321}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recU_2340802_2341422_1 recU] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigM]] {{PubMed\|18179421}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** GP891 (''recU''::''cat''), available in [[Jörg Stülke]]'s lab
−	** 1A895 (no resistance), {{PubMed\|16020779}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~== Reviews ==~~
−	~~<pubmed> 22933559 9442895</pubmed>~~
−	~~== Original publications ==~~
−	~~<pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 24792171 25939832</pubmed>~~
−
−	~~==RecU in other organisms==~~
−	~~<pubmed>23356868 </pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 1: / Line 1: @@
-* '''Description:''' Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities, equivalent to ''E. coli'' RecU<br/><br/>
+* '''Description:''' Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities, equivalent to ''E. coli'' RecU, required for efficient survival and replication restart after replication-transcription conflicts<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 147: / Line 147: @@
 <pubmed> 22933559 9442895</pubmed>
 == Original publications ==
-<pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 24792171 </pubmed>
+<pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 24792171 25939832</pubmed>
 ==RecU in other organisms==
 <pubmed>23356868 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 147: / Line 147: @@
 <pubmed> 22933559 9442895</pubmed>
 == Original publications ==
-<pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 </pubmed>
+<pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 24770420 24792171 </pubmed>
 ==RecU in other organisms==
 <pubmed>23356868 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 16:47, 5 May 2014
Line 86:		Line 86:

	* '''Effectors of protein activity:'''		* '''Effectors of protein activity:'''
		+	** [[RecU]]-mediated Holliday junction resolution is stimulated by [[RuvB]]-ATP {{PubMed\|24770420}}

	* '''[[SubtInteract\|Interactions]]:'''		* '''[[SubtInteract\|Interactions]]:'''

@@ Line 69: / Line 69: @@
 * '''Catalyzed reaction/ biological activity:'''
 * '''Protein family:'''
@@ Line 89: / Line 90: @@
 ** form dimers {{PubMed|21600217}}
 ** [[RecA]]-[[RecU]] {{PubMed|16024744}}
-** [[RecU]]-[[RuvB]] {{PubMed|21600217}}
+** [[RecU]]-[[RuvB]] {{PubMed|24770420,21600217}}
 * '''[[Localization]]:'''

@@ Line 12: / Line 12: @@
 |style="background:#ABCDEF;" align="center"| '''Product''' || Holliday junction resolvase
 |-
-|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]  and [[chromosome segregation]]
+|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]  and chromosome segregation
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310 recU]

@@ Line 55: / Line 55: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/recU-ponA.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1ZP7 1ZP7]

@@ Line 1: / Line 1: @@
-* '''Description:''' DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities <br/><br/>
+* '''Description:''' DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[RecA]] activities, equivalent to ''E. coli'' RecU<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 79: / Line 79: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 112: / Line 112: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recU_2340802_2341422_1 recU] {{PubMed|22383849}}
-* '''Sigma factor:'''  [[SigM]] {{PubMed|18179421}}
+* '''[[Sigma factor]]:'''  [[SigM]] {{PubMed|18179421}}
 * '''Regulation:'''
@@ Line 123: / Line 123: @@
 * '''Mutant:'''
-** GP891 (''recU''::''cat''), available in [[Stülke]] lab
+** GP891 (''recU''::''cat''), available in [[Jörg Stülke]]'s lab
 ** 1A895 (no resistance), {{PubMed|16020779}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
@@ Line 142: / Line 142: @@
 =References=
 == Reviews ==
-<pubmed> 22933559 </pubmed>
+<pubmed> 22933559 9442895</pubmed>
 == Original publications ==
 <pubmed>9642195,16024744,15317759,11810266,19730681 , 19422832 7814321 18179421 16154091 21600217 18684995 16020779 </pubmed>

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22310 recU]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RecU RecU]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RecU RecU]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 9.511