PyrAA - Revision history

134.76.70.252 at 14:13, 17 April 2014

2014-04-17T14:13:09Z

134.76.70.252 at 09:53, 17 April 2014

2014-04-17T09:53:16Z

134.76.38.147 at 13:40, 2 April 2014

2014-04-02T13:40:36Z

134.76.70.252 at 10:44, 7 January 2014

2014-01-07T10:44:36Z

134.76.70.252 at 13:18, 16 May 2013

2013-05-16T13:18:13Z

134.76.70.252 at 10:10, 14 May 2013

2013-05-14T10:10:51Z

134.76.70.252 at 12:47, 13 May 2013

2013-05-13T12:47:17Z

134.76.70.252 at 09:30, 7 August 2012

2012-08-07T09:30:08Z

134.76.70.252 at 09:12, 19 April 2012

2012-04-19T09:12:24Z

134.76.70.252 at 08:37, 13 April 2012

2012-04-13T08:37:34Z

← Older revision		Revision as of 14:13, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' carbamoyl-phosphate synthetase (glutaminase subunit) <br/><br/>~~	+	{{#bank: PyrAA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''pyrAA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| carbamoyl-phosphate synthetase (glutaminase subunit)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| pyrimidine biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15510 pyrAA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pyrAA pyrAA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 39 kDa, 5.818~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1092 bp, 364 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[pyrC]]'', ''[[pyrAB]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15510 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:pyrAA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrAA_1622657_1623751_1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:pyrAA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15510]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−
−
−	~~<br/><br/><br/><br/><br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis/ acquisition of nucleotides]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[PyrR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU15510
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15510&redirect=T BSU15510]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pyrRPBCAAABKDFE.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10715]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' 2 ATP + L-glutamine + HCO<sub>3</sub><sup>-</sup> + H<sub>2</sub>O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
−
−	* '''Protein family:''' carA family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[CarA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15510&redirect=T BSU15510]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P25993 P25993]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15510]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.5.5 6.3.5.5]
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrAA_1622657_1623751_1 pyrAA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed]
−
−	* '''Regulation:'''
−	** induced in the absence of uridine nucleotides ([[PyrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[PyrR]]: [[RNA switch]], transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) [http://www.ncbi.nlm.nih.gov/sites/entrez/8955403 PubMed]
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 933 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 5768 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed>8206849,1709162,, </pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 09:53, 17 April 2014
Line 123:		Line 123:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 933 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 5768 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pyrRPBCAAABKDFE.html]
@@ Line 95: / Line 96: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15510 pyrAA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pyrAA pyrAA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.818

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrAA_1622657_1623751_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pyrAA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrAA_1622657_1623751_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pyrAA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15510]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pyrC]]'', ''[[pyrAB]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15510 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15510 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:pyrAA_context.gif]]

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''

@@ Line 100: / Line 100: @@
 * '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed]
-* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed]
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrAA_1622657_1623751_1 pyrAA] {{PubMed|22383849}}
 * '''Regulation:'''