PtsG - Revision history

134.76.70.252 at 10:01, 17 April 2014

2014-04-17T10:01:36Z

Show changes

134.76.38.147 at 13:33, 2 April 2014

2014-04-02T13:33:58Z

134.76.70.252 at 10:38, 7 January 2014

2014-01-07T10:38:47Z

134.76.70.252 at 16:07, 11 November 2013

2013-11-11T16:07:43Z

Jstuelk: /* Biological materials */

2013-09-03T08:29:37Z

‎Biological materials

Jstuelk at 08:22, 3 September 2013

2013-09-03T08:22:13Z

134.76.70.252 at 13:08, 16 May 2013

2013-05-16T13:08:00Z

134.76.70.252 at 10:03, 14 May 2013

2013-05-14T10:03:56Z

134.76.70.252 at 12:41, 13 May 2013

2013-05-13T12:41:09Z

134.76.70.252 at 08:59, 7 August 2012

2012-08-07T08:59:23Z

@@ Line 64: / Line 64: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]
@@ Line 106: / Line 107: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1AX3 1AX3] (IIA domain),  [http://www.rcsb.org/pdb/explore.do?structureId=1GPR 1GPR] (IIA domain),  IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=56258 NCBI], NMR IIA domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=55395 NCBI]

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsG PtsG]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ptsG ptsG]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 75,3 kDa, 5.40

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890 ptsG]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PtsG PtsG]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsG PtsG]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''

@@ Line 137: / Line 137: @@
 =Biological materials =
-* '''Mutants:''' all available in [[ Jörg Stülke]]'s lab
+* '''Mutants:''' all available in [[Jörg Stülke]]'s lab
 ** QB5435 (cat) {{PubMed|11902727}}
 ** QB5436 (spc)
@@ Line 145: / Line 145: @@
 * '''Expression vector:'''
-** pGP123 (domains BA, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
+** pGP123 (domains BA, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
-** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
+** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
-** pGP428 (EIIB, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
+** pGP428 (EIIB, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
-** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[ Jörg Stülke]]'s lab
+** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
-** pGP34 ([[pAC5]]) {{PubMed|11902727}}, available in [[ Jörg Stülke]]'s lab
+** pGP34 ([[pAC5]]) {{PubMed|11902727}}, available in [[Jörg Stülke]]'s lab
-** pGP66 ([[pAC7]]) {{PubMed|11902727}}, available in [[ Jörg Stülke]]'s lab
+** pGP66 ([[pAC7]]) {{PubMed|11902727}}, available in [[Jörg Stülke]]'s lab
-** pGP606 (mutant terminator, [[pAC6]]), available in [[ Jörg Stülke]]'s lab
+** pGP606 (mutant terminator, [[pAC6]]), available in [[Jörg Stülke]]'s lab
 ** pGP532 ([[pAC7]]), available in [[ Jörg Stülke]]'s lab
-** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[ Jörg Stülke]]'s lab
+** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[Jörg Stülke]]'s lab
-** series of RAT mutants are available in [[pAC6]], available in [[ Jörg Stülke]]'s lab
+** series of RAT mutants are available in [[pAC6]], available in [[Jörg Stülke]]'s lab
 * '''GFP fusion:'''

@@ Line 41: / Line 41: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 127: / Line 123: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsG_1457187_1459286_1 ptsG] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|11902727}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11902727}}
 * '''Regulation:'''
@@ Line 141: / Line 137: @@
 =Biological materials =
-* '''Mutants:''' all available in [[Stülke]] lab
+* '''Mutants:''' all available in [[ Jörg Stülke]]'s lab
-** QB5435 (cat)
+** QB5435 (cat) {{PubMed|11902727}}
 ** QB5436 (spc)
 ** QB5445 (erm)
-** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)
+** GP777 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette), {{PubMed|22722928}}
-** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette)
+** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette),  {{PubMed|22722928}}
 * '''Expression vector:'''
-** pGP123 (domains BA, in [[pWH844]]), available in [[Stülke]] lab
+** pGP123 (domains BA, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
-** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[Stülke]] lab
+** pGP141 (domains BA, mut: H620D, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
-** pGP428 (EIIB, in [[pWH844]]), available in [[Stülke]] lab
+** pGP428 (EIIB, in [[pWH844]]), available in [[ Jörg Stülke]]'s lab
-** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
+** pGP437(EIIA in [[pGP570]], with thrombin cleavage site), available in [[ Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
-** pGP34 ([[pAC5]]), available in [[Stülke]] lab
+** pGP34 ([[pAC5]]) {{PubMed|11902727}}, available in [[ Jörg Stülke]]'s lab
-** pGP66 ([[pAC7]]), available in [[Stülke]] lab
+** pGP66 ([[pAC7]]) {{PubMed|11902727}}, available in [[ Jörg Stülke]]'s lab
-** pGP606 (mutant terminator, [[pAC6]]), available in [[Stülke]] lab
+** pGP606 (mutant terminator, [[pAC6]]), available in [[ Jörg Stülke]]'s lab
-** pGP532 ([[pAC7]]), available in [[Stülke]] lab
+** pGP532 ([[pAC7]]), available in [[ Jörg Stülke]]'s lab
-** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[Stülke]] lab
+** series of promoter deletions are available in [[pAC5]] and [[pAC6]], available in [[ Jörg Stülke]]'s lab
-** series of RAT mutants are available in [[pAC6]], available in [[Stülke]] lab
+** series of RAT mutants are available in [[pAC6]], available in [[ Jörg Stülke]]'s lab
 * '''GFP fusion:'''

@@ Line 33: / Line 33: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsG_1457187_1459286_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ptsG_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsG_1457187_1459286_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ptsG_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glcT]]'', ''[[ptsH]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13890 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13890 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13890 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13890 DNA_with_flanks]
 |-
 |-

@@ Line 13: / Line 13: @@
 |-
 |style="background:#ABCDEF;" align="center"|'''Function''' || glucose transport and phosphorylation, control of [[GlcT]] activity
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PtsG PtsG]